Title: Standard operating procedure for determination of microbial colonies (cfu) from a petri plates during water and environmental monitoring by using colony counter.

 

1.0       OBJECTIVE:

To lay down a procedure for determination of microbial colonies (cfu) from a petri plates during water and environmental monitoring by using colony counter.

2.0       SCOPE:

This SOP shall be applicable for counting of colonies (cfu) to digital colony counter installed in microbiology area of Q.C Lab.

3.0       RESPONSIBILITY:

3.1        Doing: Q.C Analyst/ Microbiologist.

3.2        Checking: Q.C-Manager.

4.0       ACCOUNTABILITY:

4.1        Head of the Q.C Department.

5.0       MATERIAL AND EQUIPMENT:

5.1        Digital colony counter.

6.0       PROCEDURE:

6.1        Make sure that the instrument is clean.

6.2        Operate the apparatus according to SOP.

6.3    Put petri dish one by one on the wolffhuegel grid, adjust the magnifying glass, and switch on illumination.

6.4     Ensure that the apparatus digital display is reset.

6.5    Select the square and count one by one each colony by marking with auto marker pen.

6.6    Record the total number of cfu count from display in annexure-1.

6.7    Similarly, perform test on all petri dishes. And note down colonies in annexure-1.

6.8    Switched off after use and clean it,

7.0       REVISION LOG:

Revision No.

Effective Date

Reason

00

 

New SOP

 

8.0       REFERENCES:

8.1                                                                                                                                                                                        Not Applicable.

9.0       ANNEXURES:

Annexure 1: Observations of colonies counting by using digital colony counter.

 

Annexure: 1

Observations of colonies counting by using digital colony counter

Observations of colonies counting by using digital colony counter

Date: __________________

Instrument: _____________________

No. of petri dishes: _______________

Culture performance history:

 

Petri dishes checking date on colony counter:

 

 

Sr. #

Petri dish collecting from area

(environmental/ water monitoring)

No. of Colonies displayed on screen

Remarks

 

1.    

 

 

 

2.    

 

 

 

3.    

 

 

 

4.    

 

 

 

5.    

 

 

 

6.    

 

 

 

 

Results: ________________________________________________________________

________________________________________________________________________

Performed by/ sign

Checked by/ sign

 

 

 

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