PHARMACEUTICAL QC · INTERACTIVE LEARNING
HPLC Virtual Lab
● READY
Hypothetical three-analyte mixture
Hypothetical three-analyte mixture
Pump READYFlow 1.00 mL/minPressure — barOven 30 °CDetector 254 nmColumn inlet 40% B
Compound ACompound BCompound C
0.00 / 12.00 min
Ready · Select a component or start an injection.
| Analyte | tR (min) | Area (mAU·min) | Height (mAU) | w½ (min) | T at 5% | N (w½) | Rs (w½) |
|---|
Method & sample setup
Gradient program
Time (min)B (%)A (%)
Dwell volume 0.30 mL · linear rampsSolvent system
Hypothetical neutral analytes, fixed 4.6 mm internal diameter. Selectivity, viscosity and UV spectra are illustrative. Gradient retention uses a lumped composition model; axial solvent propagation, pH and buffer effects are not modeled. Method changes require a fresh run.
Troubleshooting · Normal
STEP 1 / 15
No completed injections · %RSD requires at least 2 replicates.
T = W0.05 / (2f); N = 5.54(tR/w½)²; Rs = 1.18ΔtR/(w½,1 + w½,2). Metrics use baseline-corrected individual simulated components. Unresolved, split or incomplete peaks require review. This is not a validated integration algorithm.
Educational model · simulated data · 1× = 0.20 analysis min/sBinary pump · UV detector · post-column volume 0.04 mL
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