SOP For Calibration of UV Cabinet

SOP For calibration of UV cabinet is described in this post which you can follow in the section of the General Industry SOP.

OBJECTIVE

To calibrate the equipment for reliable and accurate results.

SCOPE

This procedure is applicable to the UV Cabinet, installed in the quality control department.

RESPONSIBILITY

QC Analyst

ACCOUNTABILITY

Manager Quality Control

PROCEDURE

For 254 nm

• Prepare a 0.04% w/v solution of sodium salicylate in ethanol (95%)
• Apply 5µl of this solution on Silica gel G with a diameter of about 5mm and examine visually.
• The distance between the lamp and the plate under examination used in a Pharmacopeial test should not exceed the distance used to carry out the above test.
• The lamp should be capable of revealing without doubt a standard spot of sodium salicylate.

For 366 nm

• Prepare a 0.2% w/v solution of sodium salicylate in ethanol (95%)
• Apply 5µl of this solution on Silica gel with a diameter of about 5mm and examine visually.
• The distance between the lamp and the plate under examination used in a Pharmacopeial test should not exceed the distance used to carry out the above test.
• The lamp should be capable of revealing without doubt a standard spot of sodium salicylate.

Frequency: Once in 3 months.