SOP For Calibration of Column Oven

SOP For calibration of column oven is described in this post which you can follow in the section of the General Industry SOP.


To provide a standard operating procedure for the calibration of HPLC Column Oven.
This procedure is applicable to all HPLC Column Oven in QC Lab.
QC Analyst
Manager Quality Control
  • Keep the sensor probe of reference calibrated digital thermometer inside the HPLC Column Oven keeps its position in about center of the oven chamber.
  • Set the oven temp. at 25 °C.
  • Let the oven chamber to achieve set temp. Allow to stabilize it for at least 5 min.
  • After 5 minutes, observe the temp. in ref. thermometer.
  • Record the value of temp. shown on instrument display and ref. the thermometer.
  • Keep minimum five minutes interval for first and second reading.
  • Then set the oven temp. 40°C, 50°C, 60°C and 70°C each one by one and follow the procedure as mentioned above.
Frequency: Once in a year.
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