Preparation Of Cleansing Agents For Glassware Washing

 

1.0  OBJECTIVE:

To lay down the preparation methods of the different cleansing agents for cleaning of glassware.

2.0  SCOPE:

This procedure is applicable to clean the glassware used for analysis installed in Quality control Lab or Microbiology Lab in Q.C area.

3.0  RESPONSIBILITY:

3.1  Doing: Laboratory Assistant/ Workman.

3.2  Checking: Technical Assistant/ Executive.

4.0  ACCOUNTABILITY:

4.1  Head of the Q.C Department.

5.0  PROCEDURE:

5.1  General cleaning agents:

5.1.1        Cleansing agents for daily or after every use usually used are:

5.1.1.1  Synthetic detergent solutions like 0.1% detergent solution (prepared by taking 1.0ml of teepol in 1000.0ml of purified water).

5.1.1.2  Alkaline cleansing agent such as trisodium phosphate.

5.1.1.3  2% Liquid soap solution:

5.1.1.3.1        Measure 20.0ml of soap solution and add it to a container containing 980.0ml of purified water and stir it well.

5.1.1.4  0.5% Labolene:

5.1.1.4.1        Measure 5.0ml of Laboleneand add it to a container containing 995.0ml of purified water and stir it well.

5.1.1.4.2        For better results use hot water.

5.2  Special cleaning agents:

5.2.1        Hot nitric acid:

5.2.1.1  Transfer laboratory grade nitric acid in beaker.

5.2.1.2  Heat it on burner under fuming hood.

5.2.1.3  And ready for use.

5.2.2        Chromic acid mixture:

5.2.2.1  A very effective cleaning fluid for removing organic matter from glass without heating is by treatment with a chromic acid mixture.

5.2.2.2  Prepared by dissolving 200.0g of sodium dichromate in about 100.0ml of purified water.

5.2.2.3  Cool it in ice-bath.

5.2.2.4  Add slowly to it 1500.0ml of sulphuric acid with stirring.

5.2.2.5  The mixture should be prepared in a hard, borosilicate glass beaker and safety glasses must be worn during the addition of acid.

5.2.2.6  Chromic acid is extremely corrosive and hygroscopic, and should be stored in glass-stoppered bottles in a safe place.

5.2.2.7  Crystalline chromic acid tends to separate from the mixture on standing, and may be removed by decantation.

5.2.2.8  When the mixture acquires a green colour, it should be discarded under continuously flowing water.

5.2.2.9  Precautions:

5.2.2.9.1        Safety glasses must be worn during the addition of acid.

5.2.2.9.2        Prepare mixture in hard borosilicate glass beaker.

5.2.2.9.3        Stored in glass-stoppered bottle in a safe place.

5.2.2.9.4        Prolonged rinsing requires when glass treated with chromic acid mixture.

5.2.2.9.5        It should not be used for cleaning calibrated containers used for optical measurements.

5.2.2.9.6        All glassware should be finally rinsed with purified water before use.

5.2.3        Commercial grade acetone:

5.2.3.1  For markers stains removal.

6.0  REVISION LOG:

Revision No.

Effective Date

Reason

00

 

New SOP

7.0  REFERENCES:

7.1  Not Applicable.

8.0  ANNEXURES:

Annexure 1: Glassware cleaning solution preparation record.

 

 

 

 

 

 

 

 

 

 

 

 

 

Annexure: 1

Glassware cleaning solution preparation record

GLASSWARE CLEANING SOLUTION PREPARATION RECORD

Date: ___________________

Name of the glassware cleaning solution:

 

Date of preparation:

 

Use before:

 

 

Method of preparation:

Take _______ml. purified water in clean and dry S.S. container or suitable and add ______ml/g of cleaning solution and mix well.

Put label on container having details of name of cleaning solution, date of preparation, use before and prepared by.

 

 

 

 

 

 

 

Prepared by (sign/date):

Checked by (sign/date):

 

 

 

9.0  ABBREVIATIONS:

Abbreviation

Expanded Form

S

Standard operating procedure

&

And

%

percentage

 


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