In this post, Standard Operating Procedure is given of Disinfectant Solutions Preparation in Pharmaceuticals Microbiology LABs.

SOP For Disinfectant Solution Preparation Objective:

This procedure has been established to describe the preparation of different disinfectants to be used in Microbiology Laboratory.  

Scope:    

This procedure is applicable to a Microbiological Lab in a Quality Control Department.

Responsibility

Microbiologist

Accountability:

Manager Quality Control 

Procedure:

Disinfectant Solution: 

Class of Disinfectant

Disinfectant Name

Concentration

Category

Aldehyde

Glutaraldehyde

3.5%

A

Oxidizing agent

Hydrogen Peroxide H2O2

7%

A

Oxidizing agent

Hydrogen Peroxide H2O2

3%

B

Alcohol

Isopropyl Alcohol (IPA)

70%

A

Phenolic Acids

Phenol

5%

A

Phenolic Acids

Phenol

2.5%

B

Alcohol+ oxidizing agent

IPA+H2O2

70%+ 3%

C

Alcohol+ Phenolic Acids

IPA+ Phenol

70%+ 2.5%

C

Oxidizing agent+ Phenolic Acids

H2O2+ Phenol

3%+ 2.5%

C


I. Glutaraldehyde (3.5%):

For preparing 100ml of 3.5% glutaraldehyde solution take 93ml of phosphate buffer pH 7 and place it on continuous stirring and  add 7ml of dropwise glutaraldehyde in it. Heat the solution until clear solution form. Filter the final solution and transfer it to the ss container and then transfer it to the storage area and label it properly.


Sr #

Qty of Disinfectant

Qty of Solvent

Final Volume

Final Concentration

1.

7ml

93ml

100ml

3.5%

2.

70ml

930ml

01L

3.5%

 3.

700ml

9300ml

10L

3.5%


II.   Hydrogen Peroxide (H2O2) 7%:

For preparing 100ml of 7% hydrogen peroxide solution, take 20ml of H2O2 in 80ml of distilled water if not available the highly purified RO water. Filter the final solution and transfer it to the ss container and then transfer it to the storage area and label it properly.


Sr #

Qty of Disinfectant

Qty of Solvent

Final Volume

Final Concentration

1.       

20ml

80ml

100ml

7%

2.       

200ml

800ml

01L

7%

3.       

02L

08ml

10L

7%


III.   Hydrogen Peroxide (H2O2) 3%:

For preparing 100ml of 3% hydrogen peroxide solution, take 8.57ml of H2O2 in 91.43ml of distilled water if not available the highly purified RO water. Filter the final solution and transfer it to the ss container and then transfer it to the storage area and label it properly.

Sr #

Qty of Disinfectant

Qty of Solvent

Final Volume

Final Concentration

1.       

8.57ml

91.43ml

100ml

3%

2.       

85.7ml

914.3ml

01L

3%

3.       

857L

9143ml

10L

3%


IV.  Isopropyl Alcohol (IPA) 70%:

For preparing 100ml of 70% IPA solution, take 70ml of IPA in 30ml of distilled water if not available the highly purified RO water. Filter the final solution and transfer it to the ss container and then transfer it to the storage area and label it properly.

Sr #

Qty of Disinfectant

Qty of Solvent

Final Volume

Final Concentration

1.       

70ml

30ml

100ml

70%

2.       

700ml

300ml

01L

70%

3.       

07L

03L

10L

70%


V.  Phenol (5%)

For preparing 100ml of 5% phenol solution, take 5g of phenol and heat it to convert the crystals into liquid form and then add in 100ml of distilled water if not available the highly purified RO water. Filter the final solution and transfer it to the ss container and then transfer it to the storage area and label it properly.

Sr #

Qty of Disinfectant

Qty of Solvent

Final Volume

Final Concentration

1.       

5g

100ml

100ml

5%

2.       

50g

01L

01L

5%

3.       

500g

10L

10L

5%


VI.   Phenol (2.5%)

For preparing 100ml of 2.5% phenol solution, take 2.5g of phenol and heat it to convert the crystals into liquid form and then add in 100ml of distilled water if not available the highly purified RO water. Filter the final solution and transfer it to the ss container and then transfer it to the storage area and label it properly.