SOP For Disinfectant Solution Preparation

In this post, Standard Operating Procedure is given of Disinfectant Solutions Preparation in Pharmaceuticals Microbiology LABs.

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This procedure has been established to describe the preparation of different disinfectants to be used in Microbiology Laboratory.  

Scope:    

This procedure is applicable to a Microbiological Lab in a Quality Control Department.

Responsibility: 

Microbiologist

Accountability:

Manager Quality Control 

Procedure:

Disinfectant Solution: 

Class of Disinfectant	Disinfectant Name	Concentration	Category
Aldehyde	Glutaraldehyde	3.5%	A
Oxidizing agent	Hydrogen Peroxide H2O2	7%	A
Oxidizing agent	Hydrogen Peroxide H2O2	3%	B
Alcohol	Isopropyl Alcohol (IPA)	70%	A
Phenolic Acids	Phenol	5%	A
Phenolic Acids	Phenol	2.5%	B
Alcohol+ oxidizing agent	IPA+H2O2	70%+ 3%	C
Alcohol+ Phenolic Acids	IPA+ Phenol	70%+ 2.5%	C
Oxidizing agent+ Phenolic Acids	H2O2+ Phenol	3%+ 2.5%	C

I. Glutaraldehyde (3.5%):

For preparing 100ml of 3.5% glutaraldehyde solution take 93ml of phosphate buffer pH 7 and place it on continuous stirring and  add 7ml of dropwise glutaraldehyde in it. Heat the solution until clear solution form. Filter the final solution and transfer it to the ss container and then transfer it to the storage area and label it properly.

Sr #	Qty of Disinfectant	Qty of Solvent	Final Volume	Final Concentration
1.	7ml	93ml	100ml	3.5%
2.	70ml	930ml	01L	3.5%
3.	700ml	9300ml	10L	3.5%

II.   Hydrogen Peroxide (H₂O₂) 7%:

For preparing 100ml of 7% hydrogen peroxide solution, take 20ml of H₂O₂ in 80ml of distilled water if not available the highly purified RO water. Filter the final solution and transfer it to the ss container and then transfer it to the storage area and label it properly.

Sr #	Qty of Disinfectant	Qty of Solvent	Final Volume	Final Concentration
1.	20ml	80ml	100ml	7%
2.	200ml	800ml	01L	7%
3.	02L	08ml	10L	7%

III.   Hydrogen Peroxide (H₂O₂) 3%:

For preparing 100ml of 3% hydrogen peroxide solution, take 8.57ml of H₂O₂ in 91.43ml of distilled water if not available the highly purified RO water. Filter the final solution and transfer it to the ss container and then transfer it to the storage area and label it properly.

Sr #	Qty of Disinfectant	Qty of Solvent	Final Volume	Final Concentration
1.	8.57ml	91.43ml	100ml	3%
2.	85.7ml	914.3ml	01L	3%
3.	857L	9143ml	10L	3%

IV.  Isopropyl Alcohol (IPA) 70%:

For preparing 100ml of 70% IPA solution, take 70ml of IPA in 30ml of distilled water if not available the highly purified RO water. Filter the final solution and transfer it to the ss container and then transfer it to the storage area and label it properly.

Sr #	Qty of Disinfectant	Qty of Solvent	Final Volume	Final Concentration
1.	70ml	30ml	100ml	70%
2.	700ml	300ml	01L	70%
3.	07L	03L	10L	70%

V.  Phenol (5%)

For preparing 100ml of 5% phenol solution, take 5g of phenol and heat it to convert the crystals into liquid form and then add in 100ml of distilled water if not available the highly purified RO water. Filter the final solution and transfer it to the ss container and then transfer it to the storage area and label it properly.

Sr #	Qty of Disinfectant	Qty of Solvent	Final Volume	Final Concentration
1.	5g	100ml	100ml	5%
2.	50g	01L	01L	5%
3.	500g	10L	10L	5%

VI.   Phenol (2.5%)

For preparing 100ml of 2.5% phenol solution, take 2.5g of phenol and heat it to convert the crystals into liquid form and then add in 100ml of distilled water if not available the highly purified RO water. Filter the final solution and transfer it to the ss container and then transfer it to the storage area and label it properly.

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Class of Disinfectant	Disinfectant Name	Concentration	Category
Aldehyde	Glutaraldehyde	3.5%	A
Oxidizing agent	Hydrogen Peroxide H2O2	7%	A
Oxidizing agent	Hydrogen Peroxide H2O2	3%	B
Alcohol	Isopropyl Alcohol (IPA)	70%	A
Phenolic Acids	Phenol	5%	A
Phenolic Acids	Phenol	2.5%	B
Alcohol+ oxidizing agent	IPA+H2O2	70%+ 3%	A
Alcohol+ Phenolic Acids	IPA+ Phenol	70%+ 2.5%	C
Oxidizing agent+ Phenolic Acids	H2O2+ Phenol	3%+ 2.5%	C