Microscope Calibration SOP



1.0  OBJECTIVE:
To lay down the calibration procedure of Microscope.
2.0  SCOPE:
This shall be applicable to the Microscope of Microbiology Laboratory.
3.0  RESPONSIBILITY:
3.1  Technical Assistant/ Helper.
3.2  Q.C Analyst/ Production Pharmacist.
4.0  ACCOUNTABILITY:
4.1  Manager-Quality Control/ Manager-Production.
5.0  MATERIAL AND EQUIPMENT:
5.1  Microscope.
6.0  PROCEDURE:
6.1  Operate the microscope according to the SOP No. BM/QCEO/SOP016-00.
6.2  Place the stage micrometer (least count 0.01mm) on the slide holder stage.
6.3  Replace the eye piece of microscope with the oculometer.
6.4  Fix the first mark of oculometer with the first mark of stage micrometer using different magnifying lens (10x, 40x and 100x).
6.5  Calculate the least count oculometer using formula given below for each (x) of objective.
Least count of oculometer (μm) = ______No. of marks of stage micrometer x 0.01 x1000_______
No. of marks of oculometer matched with stage micrometer.
6.6  Record result as per annexure-1.
6.7  Record the result on the “Calibration Record”, “Calibration Log Book” & paste the “Calibration Status Sticker” on Microscope.
6.8  Frequency:
6.8.1        Yearly.
7.0  REVISION LOG:
Revision No.
Effective Date
Reason
00

New SOP
8.0  REFERENCES:
8.1  Not Applicable.
9.0  ANNEXURES:
Annexure 1: Calibration record of Microscope.

Annexure: 1
Calibration record of Microscope
CALIBRATION RECORD OF MICROSCOPE
Calibration date
Date of last calibration done
Next due for calibration



INSTRUMENT DETAILS:
Name of equipment 
Frequency
Microscope  
Yearly

Sr. #
Objective used
No. of marks of oculometer matched with stage micrometer
No. of marks of micrometer matched with oculometer
Least count of oculometer
 (μm)
































Conclusion: Satisfactory/ Not satisfactory.

Calibrated by/date
Checked by/date




10.0    ABBREVIATIONS:
Abbreviation
Expanded Form
SOP
Standard operating procedure
&
And
No.
Number  
Ltd.
Limited
Q.C
Quality control
Q.A
Quality assurance
QCEB
Quality control equipment calibration
F
Format
mm
Millimeter
μm
Micrometer



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