SOP For calibration of TOC analyzer is described in this post which you can follow in the section of the General Industry SOP.

Objective

To check the performance of Total Organic Carbon Analyzer.

Scope

This procedure is applicable to calibrate Total Organic Carbon Analyzer.

Responsibility

QC Analyst

Accountability

Manager Quality Control

Procedure

  • Clean the instrument with a dry cotton cloth.

Performance check of TOC

  • Dissolve 0.5950 gm. of sucrose in 500 ml of water to get 500 ppm of carbon and further dilute with water to get final concentration 250 ppb, 500 ppb and 750 ppb of carbon.
  • Dissolve 0.875 gm NaHCO3 and 1.1025 gm. of Na2CO3 in 500 ml of water to get 500 ppm IC standard and further dilute with waters to get final concentration 250 ppb, 500 ppb and 750 ppb of carbon
  • Dissolve 0.5950 gm. of sucrose in 500 ml of water to get 500 ppm of carbon and further dilute with water to get final concentration 250 ppb, 500 ppb and 750 ppb of carbon. Add 1ml 2N HCl in 250, 500, 550 ppb solution.
  • Analyze the samples and check the linearity.

For system suitability

Preparation of 500 ppb benzoquinone solution 

  • Dissolve 0.375 gm. benzoquinone in 500 ml of water to get 500 ppm of carbon and further dilute and analyze with water to get final concentration 500 ppb of carbon and analyze solution as a sample.
  • Add 1ml of 2N HCl in 500 ppb benzoquinone solution.
  • Calculate the system suitability by the following calculation.

[Rss-Rw]  100X     

[Rs-Rw]

Where  

  • Rss = Mean area count of 1,4 benzoquinone solution. (500 ppb of carbon)
  • Rs = Mean area count of sucrose (500 ppb of carbon)
  • Rw  = Mean area count of pure water

Area of Limit 

a. Pure water should be more than 10,000 ( 0.25µcg carbon)

b. System suitability : Between 85% to 115% of theoretical response.

Report the results.

Performance check of SSM

Total organic carbon  

Take 2.5 mg. of Glucose for 1000 µcg std. carbon

Take 5.0 mg. of Glucose for 2000 µcg std. carbon

Take 7.5 mg. of Glucose for 3000 µcg std. carbon

Take 10.0 mg. of Glucose for 4000 µcg std. carbon

Inorganic carbon 

Take 8.85 mg. of Na2CO3 for 1000 µcg std.carbon

Take 17.7 mg. of Na2CO3 for 2000 µcg std.carbon

Take 26.55 mg. of Na2CO3 for3000 µcg std.carbon

Generate 4 point calibration curve for SSMTC and SSMIC with the point of 1000, 2000, 3000, 4000 µcg standards.

In calibration condition “Set the

1st std. concentration as 40 and AMNT 2.5mg

2nd std. concentration as 40 and AMNT 5.0mg

3rd std. concentration as 40 and AMNT 7.5mg

4th std. concentration as 40 and AMNT 10 mg

Set the range as per requirement

Press “O.K” and then press the start button.

After completion all four std. shift to original function in ‘cal curve’ screen before using.

Similarly create a calibration curve for various std.

Frequency for Total organic carbon 

For TC and IC = Once in a six month

For NPOC = Once in a month

For system suitability: Once in three month

Frequency for SSM

TC and IC = Once in a six month