Sterilization of dresses used in Sterile Work Area
1. Purpose:-
To ensure effective
sterilization of protective clothing.
2. Scope:-
This
procedure applies to sterilization process for protective dresses used when
performing sterility tests in Microbiology lab.
.
3. HSE
Statement:-
Asbestos
Gloves
4. Responsibilities:-
i)
Manager Quality Control is responsible
to ensure that procedure & formats are followed entirely as approved.
ii)
Microbiologist is responsible to perform the sterilization
process.
5. Materials:-
5.1 Jump suit
5.2 Shoe covers
5.3 Pairs of Gloves
5.4 Butter Paper
5.5 Masking Tape
5.6 Autoclave
6. Definitions:-
6.1. Sterilization:-
Rendering
an object or substance free of viable microbes but not of microbial toxins, such as, endotoxinby destroying or
removing all viable microorganisms, including viruses. An object cannot be slightly
sterile or almost sterile- it is either sterile or not sterile
7. Flow Chart:-
8.1.1 The jump suit set used in
Microbiology Lab, comprises of five separate pieces.
a. Jump suit -
One
b. Head Cover - One
c. Mask - One
d. Shoe Cover - One pair
e. Gloves - One pair
8.1.2 Wrap all
the above components except pair of gloves in a butter paper.
8.1.3 Place
parachute jump suit. If required fold 1/3 of the arms and the legs of the jump
suit inside out.
8.1.4 Wrap
gloves separately in butter paper.
8.1.5
Wrap the dress by folding and sealing the butter paper with masking tap Now
take another butter paper sheet and place the wrapped suit in inverted position
on the butter sheet.
8.1.6 Similarly wrap this paper as done before.
8.1.7 Mark on one side having jump suit upward to open the dress from
this side. Also mark with date.
8.1.8 Place the
wrapped jump suits set in nylon bag, close the bag and button or tie.
8.1.9 Sterilize
the nylon bag containing jump suit set and gloves in autoclave at 121 0C
for not less then 30 minutes.
8.1.10 Use a Thermo
log – S integrator with each load that indicates completion of sterilization.
8.1.11Un- used sterile dresses should be removed from the sterile area
after 72 hour
9. Records:- Sterilization
log (Autoclave)
10. References:-
11. Distribution:-
This SOP has to be distributed in below mentioned
Departments:-
|
Sr. NO
|
Distributed to
|
Received
(Current)
|
Returned
(Obsolete)
|
|
1
|
Quality Control
Department
|
|
|
|
2
|
Quality Management
Department
|
|
|
12.
Revision History:-
Date Changes
N/A