Potentiometer Operational SOP

1.0  OBJECTIVE:

To lay down the operating procedure of Potentiometer.

2.0  SCOPE:

This shall be applicable to the Potentiometer of Quality Control Laboratory.

3.0  RESPONSIBILITY:

3.1  Technical Assistant/ Helper.

3.2  Q.C Analyst.

4.0  ACCOUNTABILITY:

4.1  Manager-Quality Control.

5.0  MATERIAL AND EQUIPMENT:

5.1  Potentiometer.

5.2  Sample quantity as specified in monograph.

5.3  Solvent as specified in monograph.

6.0  PROCEDURE:

6.1  Check that the power supply to the instrument is switched “OFF” before cleaning.

6.2  Clean the instrument according to SOP.

6.3  Check that the instrument is connected to the stabilized power supply.

6.4  Switch “ON” the main power supply.

6.5  On desktop double click on “tiamo1.2” icon. Screen show “tiamo1.2” it will initialize program and devices.

6.6  Preparation message appears. Press “OK” to all.

6.7  Four options will appear on the left side screen show. Workplace, database, method, configuration.

6.8  Click on workplace option. Four windows are seen. Run, method, report, live display 1.

6.9  Click on “Tools/manual control”. Select dosing device and go to preparation function. Press start. The burette tip should point in a waste beaker. This step rinses the burette and removes any possible air bubble.

6.10    Before doing titration check electrode input, dosing device & stirrer to be used in the method (selected for titration).

6.11    In workplace on RUN window click on METHOD to select the appropriate method.

6.12    Weigh the standard/ sample in a beaker and add the appropriate solvent, place it on the magnetic stirrer to be used & put the magnetic paddle into it. Dip appropriate electrode assembly along with dispensing tip in the beaker just above the stirrer bar.

6.13    Enter sample name, batch no. LNB Ref Analyst name & sample size (for assay also enter the equivalent weight also).

6.14    Click on START a window with sample detail will appear on the screen press OK.

6.15    Titration proceeds. After the titration is over burette goes for filling and then report appears in the report window.

6.16    As the experiment is over, remove the beaker, wash the electrode properly.

6.17    Record the observations in usage log book (Annexure-I).

6.18    Report print:

6.18.1    Click on “Data base”.

6.18.2    Open the appropriate data base.

6.18.3    Select the report to be print.

6.18.4    Click on “Tools” on menu.

6.18.5    Open “Report template”.

6.18.6    For report of “Blank” determination select titer template.

6.18.7    For report of “Titer” determination select titer/ titer with statistics.

6.18.8    For “Short report” of assay determination select template 1 & for “Detail report” of assay determination select template 2.

6.18.9    After selecting appropriate template click on “open”.

6.18.10Click on “PDF” a PDF file will be created, save it or take print out.

6.19    Precautions:

6.19.1    Check the levels of electrolytes in electrode regularly and replenish.

6.19.2    For exchange unit containing NaOH apply grease to piston every week.

7.0  REVISION LOG:

Revision No.	Effective Date	Reason
00		New SOP

8.0  REFERENCES:

8.1  Not Applicable.

9.0  ANNEXURES:

Annexure 1: Usage log book of potentiometer.

Annexure: 1

Usage log book of Potentiometer

Date	Product/ Material Name	Batch No./ Lot No.	Time		Analysis performed	Analyzed by	Checked by	Remarks
			Started at	Completed at

10.0    ABBREVIATIONS:

Abbreviation	Expanded Form
SOP	Standard operating procedure
&	And
No.	Number
Ltd.	Limited
Q.C	Quality control
Q.A	Quality assurance
QCEO	Quality control equipment operations
Ml	Milliliter
Mv	Millivolts
Hz	Hertz
v/s	Verses