Methyl red solution Reagent Preparation SOP


Title: Standard operating procedure of Methyl red solution.
1.0  OBJECTIVE:
To lay down the procedure of Methyl red solution for Laboratory use.
2.0  SCOPE:
This shall be applicable to the Quality Control Laboratory.
3.0  RESPONSIBILITY:
3.1  Q.C Pharmacist.
4.0  ACCOUNTABILITY:
4.1  Manager-Quality Control.
4.2  Head of the Q.C Department.
5.0  MATERIAL AND EQUIPMENT:
5.1  Glassware (one 100.0ml beaker, one stirrer, one pipette).
5.2  Magnetic stirrer.
5.3  50.0mg of methyl red.
5.4  1.86ml of 0.1M sodium hydroxide.
5.5  50.0ml of alcohol R.
5.6  Purified water q.s.
6.0  PROCEDURE:
6.1  Take a beaker of 100.0ml; add 50.0mg of methyl red in it.
6.2  Add a mixture of 1.86ml of 0.1M sodium hydroxide and 50.0ml of alcohol R.
6.3  Mix it by using magnetic stirrer.
6.4  Dilute it to 100.0ml with purified water R.
7.0  REVISION LOG:
Revision No.
Effective Date
Reason
00

New SOP

8.0  REFERENCES:
8.1  The British Pharmacopoeia. Vol V. Appendix I, Official Monograph /General Reagent: 2015, pp.19-141.
9.0  ANNEXURES:
9.1  Not Applicable.
10.0    ABBREVIATIONS:
Abbreviation
Expanded Form
SOP
Standard operating procedure
&
And
No.
Number  
Ltd.
Limited
Q.C
Quality control
QCR
Quality control reagent
ml
Milliliter
mg
Milligram  
M
Molar
q.s.
Quantity sufficient
R
Reagent
Vol
Volume


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