Mechanical pipettor Calibration SOP

1.0  OBJECTIVE:

To lay down the calibration procedure of Mechanical pipettor for reliable and accurate results during mixing.

2.0  SCOPE:

This shall be applicable to the Mechanical pipettor of Quality Control Instrument Laboratory.

3.0  RESPONSIBILITY:

3.1  Technical Assistant/ Helper.

3.2  Q.C Analyst.

4.0  ACCOUNTABILITY:

4.1  Manager-Quality Control.

5.0  MATERIAL AND EQUIPMENT:

5.1  Mechanical pipettor.

6.0  PROCEDURE:

6.1  Maintain the Mechanical pipettor according to the SOP

6.2  Operate the Mechanical pipettor according to the SOP

6.3  Calibration should be done every 3 month with weight variation.

6.4  Different set of volumes should be taken and linearity should be performed.

6.5  Attach the micropipette tips to micropipette. The desired volume is set and water shall be aspirated. Dispensed into dry beaker and weighed repeat for 10 times. The mean of the weights are converted into μl using the following formula:

Volume = _____Weight of water sample._____

             Density of water (at 25^oC)

6.6   The percentage variation can be calculated by using following formula:

Percentage variation = Actual weight – Observed weight x 100

Actual weight

6.7   Perform the calibration by suing the set volume.

6.8  Record the result on the “Calibration Record”, “Calibration Log Book” & paste the “Calibration Status Sticker” on Mechanical pipettor.

6.9  Precautions:

6.9.1     Use the analytical balance with a readability of 0.1mg.

7.0  REVISION LOG:

Revision No.	Effective Date	Reason
00		New SOP

8.0  REFERENCES:

8.1  Not Applicable.

9.0  ANNEXURES:

Annexure 1: Calibration report of Mechanical pipettor.

Annexure: 1

Calibration report of Mechanical pipettor

Calibration report of Mechanical pipettor Calibration date Date of last calibration done Next due for calibration Instrument details Instrument name Instrument make Instrument identification No. Observation Chart Sr. # Volume taken in (μl) Tolerance limit Remarks Min Max Limit: RSD of Net volume of sample NMT 1%. Conclusion: Satisfactory/ Not satisfactory. Done by/date Checked by/date

10.0    ABBREVIATIONS:

Abbreviation	Expanded Form
SOP	Standard operating procedure
&	And
No.	Number
Ltd.	Limited
Q.C	Quality control
Q.A	Quality assurance
QCEB	Quality control equipment calibration
F	Format