1.0 OBJECTIVE:
To
lay down the procedure for cleaning of Glassware.
2.0 SCOPE:
This
shall be applicable to the Glassware
of Quality Control Instrument Lab.
3.0 RESPONSIBILITY:
3.1
Technical
Assistant/ Helper.
3.2
Q.C Analyst.
4.0 ACCOUNTABILITY:
4.1
Manager-Quality
Control.
5.0 MATERIAL AND
EQUIPMENT:
5.1
Glassware.
5.2
Dry lint free
cloth (2 pieces).
5.3
Purified water.
6.0 PROCEDURE:
6.1 Cleaning
of glassware, which has contained hazardous materials, must be solely
undertaken by experienced personnel.
6.2 Most
new glassware is slightly alkaline in reaction. For precision chemical tests,
new glassware should be soaked several hours in acid water (1% solution
hydrochloric acid or nitric acid) before washing.
6.3 Wash
glassware as quickly as possible after use if it is not possible then the
articles should be allowed to soak in purified water.
6.4 General cleaning:
(Daily/ after every use)
6.4.1
Before starting
cleaning of glassware remove the labeling of marker pen with the help of
acetone. For plastic ware do not use acetone for removing marker pen labeling.
6.4.2
Wash the used
glassware properly with tap water.
6.4.3
Then scrub the
glassware with 0.1% detergent solution or any cleansing agent for regular use.
6.4.4
Special types of
precipitate material may require removal with nitric acid, aqua regia or fuming
sulphuric acid. These are very corrosive substances and should be used only
when required.
6.4.5
If needed dip all
the glassware in liquid soap for 2 hours. Remove the glassware from the liquid
soap solution. Remove the marker stains using commercial grade acetone.
6.4.6
Brush all the
possible areas of glassware.
6.4.7
Rinse all the
glassware properly with running tap water.
6.4.8
Again rinse 3 to 4
times with purified water.
6.4.9
Drain the water
from glassware.
6.4.10 Dry
the glassware in hot air oven at 60oC±5oC.
6.4.11 Remove
the glassware using gloves and cool to room temperature.
6.4.12 Now
store properly in cupboard. And ready for use.
6.4.13 For
microbiology laboratory glassware autoclave the glassware at 121oC,
15lbs pressure for validated cycle time. Plug the mouth of glassware with
non-absorbent cotton plug and wrapping the glassware carefully with butter
paper. Affix biological indicator if required.
6.4.14 Affix
labels having date of sterilization & use before on glassware.
6.4.15 After completion of cleaning affix
the “CLEANED” label on the cupboard.
6.4.16 Fill
the “Cleaning Equipment Log Book”.
6.5 Special cleaning:
(Once in a month/ whenever necessary)
6.5.1
By
using hot nitric acid:
6.5.1.1 Prepare
hot nitric acid as specified in SOP BM/QC/SOP070-00.
6.5.1.2 Wear
safety goggles & rubber gloves properly.
6.5.1.3 Rinse the glassware with hot nitric acid.
6.5.1.4 Allow it to stand overnight.
6.5.1.5 Rinse properly with tap water.
6.5.1.6 Finally rinse with purified water.
6.5.1.7 Drain the water.
6.5.1.8 Dry
the glassware in hot air oven at 60oC±5oC.
6.5.1.9 Remove
the glassware using gloves and cool to room temperature.
6.5.1.10
Now store properly
in cabinet.
6.5.2
By
using chromic acid mixture:
6.5.2.1 Prepare
chromic acid mixture as specified in SOP BM/QC/SOP070-00.
6.5.2.2 Dip
the glassware, once in a month in chromic acid mixture and keep in overnight.
6.5.2.3 Wear
safety goggles & rubber gloves properly.
6.5.2.4 Remove
the glassware carefully.
6.5.2.5 Rinse
the glassware thoroughly for prolonged time with tap water, as glass tends to
absorb chromic acid.
6.5.2.6 Rinse
the glassware finally with distilled water.
6.5.2.7 Dry
the glassware in hot air oven at 60oC±5oC.
6.5.2.8 Remove
the glassware using gloves and cool to room temperature.
6.5.2.9 Now
store properly in cabinet.
6.6 Cleaning of
specific type of glassware:
6.6.1
Pipette:
6.6.1.1 Place
pipettes tips down, in a cylinder or tall jar containing disinfectant solution
immediately after use.
6.6.1.2 Do
not drop them into the cylinder, since this may break or chip the tips and
render the pipettes useless for accurate measurements. A pad of cotton or glass
wool at the bottom of the cylinder will help to prevent breaking of the tips.
6.6.1.3 At
a convenient time, the pipettes may then be drained and placed in a cylinder or
jar of dissolved detergent or, if exceptionally dirty, in a jar of chromic acid
cleaning solution.
6.6.1.4 After
soaking of several hours, or overnight, drain the pipettes and run tap water
over and through them until all traces of dirt are removed. Soak the pipettes
in purified water for at least one hour.
6.6.1.5 Remove
from the purified water, dry outside with a cloth, shake out the water and dry
in hot air oven.
6.6.1.6 After
drying, place pipettes in a dust free drawer.
6.6.2
Burettes
(with glass stopcock):
6.6.2.1 Remove
the stopcock key and wash the burette with detergent and water.
6.6.2.2 Rinse
with tap water until all the dirt is removed. Then rinse with purified water
and dry.
6.6.2.3 Wash
the stopcock key separately. Before the stopcock key is replaced in the
burette, lubricate the joint with a small amount of lubricant. Remember those
burette stopcock keys are not interchangeable.
6.6.2.4 Always
cover burettes when not in use.
6.6.3
Gooch
crucible/ sintered glass funnel:
6.6.3.1 Fill
the gooch crucible of sintered glass funnel with hot nitric acid.
6.6.3.2 Allow
it to stand overnight.
6.6.3.3 Rinse
with raw water.
6.6.3.4 Apply
vacuum and wash the crucible with raw water.
6.6.3.5 Then
wash 3 to 4 times with purified water.
6.6.3.6 Keep
in drier.
6.6.4
Silica
crucible:
6.6.4.1 Fill
the silica crucible with nitric acid and heat it over the burner under fuming
hood.
6.6.4.2 Wash
with raw water.
6.6.4.3 Wash
with purified water.
6.6.4.4 Ignite
the crucible at 700oC temperature in a muffle furnace.
6.6.4.5 Cool
and keep in respective place.
6.6.5
Petri
plates:
6.6.5.1 Remove
agar from the petri plates with a blunt knife.
6.6.5.2 Collect
the agar in a S.S. container and autoclave at 121oC, 15lbs pressure
for 30min.
6.6.5.3 Immediately
dispose the medium by burning in the specified place or drain it in ETP.
6.6.5.4 Dip
the plates in a washtub filled with disinfectant solution.
6.6.5.5 Rinse
the plates with tap water and dip them in a washtub containing 2% soap solution
or 0.5% Labolene.
6.6.5.6 Scrub
each plate with a brush or scrubber.
6.6.5.7 Wash
each plate thoroughly with tap water to remove soap with purified water.
6.6.5.8 Finally
rinse with purified water. Keep the plates for drying in oven at 60-65oC.
6.6.5.9 After
drying pack 5 to 10 petri dishes in bundle and wrap with cellofen/ butter
paper.
6.6.5.10
Affix indicator if
required and autoclave at 121oC, 15lbs pressure for validated cycle
time.
6.6.5.11
Affix labels
having date of sterilization & use before.
6.7 Precautions:
6.7.1
After washing with
tap water rinsing with purified water must be good enough.
6.7.2
Brush must be selected
for the shape and size of the glassware for scrubbing.
6.7.3
Brushes should
always be in good condition to avoid any abrasion of the glassware.
6.7.4
It is imperative
that all soap detergents and other cleaning fluids be removed from glassware
before use. This is especially important with detergents, slight traces of
which will interfere with serological and culture reactions.
6.7.5
Always protect
clean glassware from dust by use of temporary closures or by placing in a dust
free cabinet.
6.7.6
While handling
used microbiology laboratory glassware wear disposable latex rubber hand
gloves.
6.7.7
All contaminated
glassware should be sterilized at 121oC, 15lbs pressure for
validated cycle time.
7.0 REVISION LOG:
|
Revision No.
|
Effective Date
|
Reason
|
|
00
|
|
New
SOP
|
8.0 REFERENCES:
8.1 Not Applicable.
9.0 ANNEXURES:
Annexure 1: Cleaning
record of Glassware (Cleaning
Equipment Log Book).
Annexure:
1
(Cleaning
Equipment Log Book)
Cleaning
record of Glassware
|
CLEANING
RECORD OF GLASSWARE
|
10.0
ABBREVIATIONS:
|
Abbreviation
|
Expanded Form
|
|
SOP
|
Standard operating
procedure
|
|
&
|
And
|
|
No.
|
Number
|
|
Ltd.
|
Limited
|
|
QCEC
|
Quality control Equipment
cleaning
|
|
Q.C
|
Quality control
|
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