1.0 OBJECTIVE:
To
lay down the operating procedure of Fourier transformer infrared radiation (FTIR).
2.0 SCOPE:
This
shall be applicable to the Fourier
transformer infrared radiation (FTIR) of Quality Control Instrument Laboratory.
3.0 RESPONSIBILITY:
3.1
Technical
Assistant/ Helper.
3.2
Q.C Analyst.
4.0 ACCOUNTABILITY:
4.1
Manager-Quality
Control.
5.0 MATERIAL AND
EQUIPMENT:
5.1
Fourier transformer infrared radiation (FTIR).
6.0 PROCEDURE:
6.1 “Switch
on” FTIR, Computer &
printer. Place sample plate (empty ) in sampling
compartment.
6.2 Double
click on software with the name of ²spectrum².
6.3 Login
window open, Enter login name, if
required otherwise click on ²O.K²
6.4 Spectrum
window open as graph. (X-axis, Y-axis).
6.5 BACKGROUND SCANING
6.5.1
Click on
“instrument” on tool bar.
6.5.2
Click on ²SCAN
BACK GROUND²
at first time only.
6.5.3
Scan back ground
window open, enter² FILE NAME² as BKG or other
Words &
description as²
Back ground².
6.5.4
Select “start
range”, “end range” as required for
scanning.
6.5.5
Select number of
scans (normally 6.4).
6.5.6
Click on ²
SET UP ²
in the same window.
6.5.7
New window open
with the name of ²SPECTRUM BX FT- IR
6.5.8
INSTRUMENT SET UP².
Enter in ² DESCRIPTION² if required.
6.5.9
Click on ² SCAN PARAMETER ²
in the same window.
Enter
the following.
6.5.10 Click
Beam type Box and Beam type window open.
6.5.11 Select
in ²
BACK GROUND²
Then ¢O.K¢.
6.5.12 In
Data type Base, always ¢Single¢.
6.5.13 Click
on ²UP
DATE²
6.5.14 Click
on ²O.K
².
It will be return on the ²SCAN BACK GROUND²
Window. Click on ²O.K ² Instrument start
automatically to scan back ground and open window for ²
Name ²
Enter name of file as required. i.e. BKG etc. and click on new name or
overwrite.
6.6 SAMPLE SCANING
6.6.1
Click on
instrument on tool bar. Then click on ² Scan sample²
6.6.2
Collect sample
plate from instrument sampling compartment.
6.6.3
Pour sample powder
/liquid on sample plate and press powder with spatula manually to ensure
complete filling.
6.6.4
Put sample plate
in sampling compartment carefully.
6.6.5
Put powder press
bar on sample plate and push lever down and screw the press bar to press the
powder uniformly and close the sampling compartment.
6.6.6
Click on²
instrument ²on
tool bar, then click on ² SCAN SAMPLE²
6.6.7
Scan sample window
open automatically.
Enter
the following
In
file name box write the sample name and write in description box ²SAMPLE² ²RATIO ²
appear in window as ²SELECTED²
6.6.8
Click on ²setup²
in the same window and new window appear automatically.
6.6.9
Write down in
description box (if required).
6.6.10 Click
on ²SCAN
PARAMETER² and new window open.
6.6.11 Click
on beam type box, beam type window open.
6.6.12 Select
²SAMPLE²
In this window and click ²O.K² Click on ²DATA
TYPE BASE ²
New window open and select On ²RATIO² Then click on ²O.K
²
6.6.13 Click
on²
UP DATE²
again scan sample window appear, click on ² OK²,
Instrument automatically scan spectra and name window open. Write down the name
of file and click on new file or overwrite. Both spectra ² BACK GROUND
SPECTRUM ²
and ²SAMPLE
SPECTRA²
appear on the screen. Select ²BACK GROUND ²at bottom of the
screen and press ²DELETE². Back ground spectra
disappear from the screen.
6.6.14 Select
the sample spectra with ² CURSOR ² and double click
through mouse to optimize the spectrum.
6.6.15 Click
on label peak to label the peak.
6.6.16 Click
²ABC²
to add test in spectra i.e. name of the compound etc. Click print to print the
spectra.
6.7 PRECAUTION:
6.7.1
Preserve
instrument from moisture.
6.7.2
Instrument switch
on daily Basis.
6.7.3
Always clean the
instrument with dry lint free cloth.
6.7.4
Avoid placing body
parts in the path of light in sampling chamber.
6.7.5
Clean the sampling
plate with dry and moist tissue paper, dry it before use.
6.7.6
Change desiccators
after each six month and purge with nitrogen gas.
7.0 REVISION LOG:
|
Revision No.
|
Effective Date
|
Reason
|
|
00
|
|
New
SOP
|
8.0 REFERENCES:
8.1
Not Applicable.
9.0 ANNEXURES:
Annexure 1: FTIR
Usage Logbook.
Annexure: 1
FTIR Usage Logbook
|
FTIR
USAGE LOGBOOK
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10.0
ABBREVIATIONS:
|
Abbreviation
|
Expanded Form
|
|
SOP
|
Standard operating
procedure
|
|
&
|
And
|
|
No.
|
Number
|
|
Ltd.
|
Limited
|
|
Q.C
|
Quality control
|
|
QCEO
|
Quality control
equipment operation
|
|
F
|
Format
|
|
KBr
|
Potassium bromide
|
|
FTIR
|
Fourier transforms infrared radiation
|
|
cm-1
|
Per centimeter
|
|
DRA
|
|
No comments:
Post a Comment