1.0 OBJECTIVE:
To
lay down the calibration procedure of Fourier transforms infrared radiation (FTIR).
2.0 SCOPE:
This
shall be applicable to the Fourier
transforms infrared radiation (FTIR) of Quality Control Laboratory.
3.0 RESPONSIBILITY:
3.1
Technical
Assistant/ Helper.
3.2
Q.C Analyst.
4.0 ACCOUNTABILITY:
4.1
Manager-Quality
Control.
5.0 MATERIAL AND
EQUIPMENT:
5.1
Fourier transforms infrared radiation (FTIR).
6.0 PROCEDURE:
6.1 Switch
on the system.
6.2 Set
instrument parameter as follow:
6.2.1
RESOLUTION: 2.0.
6.2.2
APODIZATION STRONG
6.2.3
RANGE: 4000-400cm-1.
6.2.4
MODE RATIO.
6.2.5
NUMBER OF SCAN.
6.3 Allow
the instrument to warm for 39 minutes.
6.4 Operate
the instrument as per operation SOP.
6.5 Open
the sample compartment cover of FTIR 1600 and place the polystyrene film in the
sample holder and close the cover.
6.6 Click
OK in the SCAN MODE.
6.7 The
spectrum of the polystyrene film is displays on the screen.
6.8 PRINT
the spectrum by going to print function.
6.9 When
the printer of the spectra is completely go to DATA and then to PEAK.
6.10
The peak data’s of
the spectra of the polystyrene film is displays on the screen.
6.11
PRINT out these
data’s by going to print function.
6.12
Compare the wave
function accuracy with the following limit.
Limit
of wave number accuracy
|
Wave
number
|
Limit
|
|
3060.0
|
(±1.5)
|
|
2849.5
|
(±1.5)
|
|
1942.9
|
(±1.5)
|
|
1601.2
|
(±1.5)
|
|
1583.0
|
(±1.5)
|
|
1154.5
|
(±1.5)
|
|
1026.3
|
(±1.5)
|
Resolution
performance of the apparatus
Record the spectrum of the
polystyrene film 0.04mm in thickness. The difference x between the percentage
transmittance at the transmission maximum A at 2870cm-1 (3.48μm) and
that at the transmission minimum B at 2849.5cm-1 (3.51μm) should
greater than 16. The difference y between the percentage transmittance at the
transmission maximum C at 1589cm-1 (5.29μm) and that at the
transmission minimum D at 1583cm-1 (5.32μm) should be greater than
12.
6.13
Record the
observation in the calibration record i.e. annexure-2.
6.14
Affix a “CALIBRATION LABEL” on the instrument.
6.15
Report any
discrepancy observed during calibration or operation of the instrument to QA
Manager and notify the defect to engineering dept./service. Affix an “UNDER MAINTENANCE”; label on the
instrument.
6.16
Frequency
of calibration:
6.16.1
Once in three
month and after each maintenance job.
7.0 REVISION LOG:
|
Revision No.
|
Effective Date
|
Reason
|
|
00
|
|
New
SOP
|
8.0 REFERENCES:
8.1 Not Applicable.
9.0 ANNEXURES:
Annexure 1: Calibration
record of Fourier Transforms
Infrared Radiation (FTIR).
Annexure: 1
Calibration
record of Fourier Transforms
Infrared Radiation (FTIR)
|
CALIBRATION
RECORD OF FOURIER TRANSFORMS
INFRARED RADIATION
OBSERVATIONS
Limit
of wave number accuracy by polystyrene film:
Resolution
performance by polystyrene film:
Remarks:
The
instrument is working satisfactorily/ not satisfactorily as per SOP.
|
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10.0
ABBREVIATIONS:
|
Abbreviation
|
Expanded Form
|
|
SOP
|
Standard operating
procedure
|
|
&
|
And
|
|
No.
|
Number
|
|
Ltd.
|
Limited
|
|
Q.C
|
Quality control
|
|
Q.A
|
Quality assurance
|
|
QCEB
|
Quality control
equipment calibration
|
|
F
|
Format
|
|
FTIR
|
Fourier transforms infrared radiation
|
|
cm-1
|
Per centimeter
|
|
mm
|
Millimeter
|
|
μm
|
Micrometer
|
|
Dept.
|
Department
|
|
%
|
Percentage
|