Crystal violet solution Reagent Preparation SOP

Title: Standard operating procedure of Crystal violet solution preparation for laboratory use.


1.0  OBJECTIVE:
To lay down the procedure of Crystal violet solution for Laboratory use.
2.0  SCOPE:
This shall be applicable to the Quality Control Laboratory.
3.0  RESPONSIBILITY:
3.1  Q.C Analyst.
4.0  ACCOUNTABILITY:
4.1  Manager-Quality Control.
4.2  Head of the Q.C Department.
5.0  MATERIAL AND EQUIPMENT:
5.1  Glassware (1 beaker, 1 pipette, 1 spatula, 1 glass rod).
5.2  Magnetic stirrer.
5.3  Analytical weighing balance.
5.4  0.5g of crystal violet R.
5.5  Anhydrous acetic acid R (q.s.).
6.0  PROCEDURE:
6.1  Firstly weigh 0.5g of crystal violet R by using analytical weighing balance operate according to SOP.
6.2  Take 100.0ml of beaker and add 0.5g of crystal violet R in it.
6.3  Add sufficient quantity of anhydrous acetic acid R.
6.4  Mix it by using magnetic stirrer.
6.5  And dilute it to 100.0ml with the same solvent.
6.6  Again mix it thoroughly by using magnetic stirrer.
6.7  Ready for use.
7.0  REVISION LOG:
Revision No.
Effective Date
Reason
00

New SOP

8.0  REFERENCES:
8.1  The European Pharmacopoeia. Vol-1. Official Monograph /Reagents: 2015, pp.425-536.
9.0  ANNEXURES:
9.1  Not Applicable.
10.0    ABBREVIATIONS:
Abbreviation
Expanded Form
SOP
Standard operating procedure
&
And
No.
Number  
Ltd.
Limited
QCR
Quality control reagent
Q.C
Quality control
ml
Milliliter
Vol
Volume
q.s
Quantity sufficient
g
Grams
R
Reagent


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