1.0 OBJECTIVE:
To
lay down the procedure of Crystal
violet solution for Laboratory use.
2.0 SCOPE:
This
shall be applicable to the Quality Control Laboratory.
3.0 RESPONSIBILITY:
3.1
Q.C Analyst.
4.0 ACCOUNTABILITY:
4.1
Manager-Quality
Control.
4.2
Head of the Q.C
Department.
5.0 MATERIAL AND
EQUIPMENT:
5.1
Glassware (1 beaker,
1 pipette, 1 spatula, 1 glass rod).
5.2
Magnetic stirrer.
5.3
Analytical
weighing balance.
5.4
0.5g of crystal
violet R.
5.5
Anhydrous acetic
acid R (q.s.).
6.0 PROCEDURE:
6.1
Firstly weigh 0.5g
of crystal violet R by using analytical weighing balance operate according to
SOP.
6.2
Take 100.0ml of
beaker and add 0.5g of crystal violet R in it.
6.3
Add sufficient
quantity of anhydrous acetic acid R.
6.4
Mix it by using
magnetic stirrer.
6.5
And dilute it to
100.0ml with the same solvent.
6.6
Again mix it
thoroughly by using magnetic stirrer.
6.7
Ready for use.
7.0 REVISION LOG:
|
Revision No.
|
Effective Date
|
Reason
|
|
00
|
|
New
SOP
|
8.0 REFERENCES:
8.1 The European Pharmacopoeia. Vol-1. Official Monograph /Reagents: 2015,
pp.425-536.
9.0 ANNEXURES:
9.1
Not Applicable.
10.0
ABBREVIATIONS:
|
Abbreviation
|
Expanded Form
|
|
SOP
|
Standard operating
procedure
|
|
&
|
And
|
|
No.
|
Number
|
|
Ltd.
|
Limited
|
|
QCR
|
Quality control reagent
|
|
Q.C
|
Quality control
|
|
ml
|
Milliliter
|
|
Vol
|
Volume
|
|
q.s
|
Quantity sufficient
|
|
g
|
Grams
|
|
R
|
Reagent
|