Copper Edetate solution Reagent Preparation SOP


Title: Standard operating procedure of Copper Edetate solution preparation for laboratory use.
1.0  OBJECTIVE:
To lay down the procedure of Copper Edetate solution for Laboratory use.
2.0  SCOPE:
This shall be applicable to the Quality Control Laboratory.
3.0  RESPONSIBILITY:
3.1  Q.C Analyst.
4.0  ACCOUNTABILITY:
4.1  Manager-Quality Control.
4.2  Head of the Q.C Department.
5.0  MATERIAL AND EQUIPMENT:
5.1  Glassware (3 beaker, 1 pipette, 1 spatula, 1 glass rod).
5.2  Magnetic stirrer.
5.3  Analytical weighing balance.
5.4  2.0ml of 2g/l solution of copper acetate R.
5.5  0.02g of copper acetate.
5.6  2.0ml of 0.1M sodium edetate.
5.7  Purified water (q.s.).
6.0  PROCEDURE:
6.1  Firstly weigh 0.02g of copper acetate by using analytical weighing balance operate according to SOP.
6.2  Prepare 0.02g/10ml solution of copper acetate R separately in beaker (i.e. equivalent to 20g/l solution of copper acetate).
6.3  In another beaker prepare 0.1M sodium edetate.
6.4  Take 50.0ml of beaker and mix 2.0ml of a 0.02g/10ml solution of copper acetate R add 2.0ml of 0.1M sodium edetate.
6.5  Mix it by using magnetic stirrer.
6.6  And dilute it to 50.0ml with purified water R.
6.7  Again mix it thoroughly by using magnetic stirrer.
6.8  Ready for use.
7.0  REVISION LOG:
Revision No.
Effective Date
Reason
00

New SOP

8.0  REFERENCES:
8.1  The European Pharmacopoeia. Vol-1. Official Monograph /Reagents: 2015, pp.425-536.
9.0  ANNEXURES:
9.1  Not Applicable.


10.0    ABBREVIATIONS:
Abbreviation
Expanded Form
SOP
Standard operating procedure
&
And
No.
Number  
Ltd.
Limited
QCR
Quality control reagent
Q.C
Quality control
ml
Milliliter
Vol
Volume


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