Title: Standard operating procedure of Copper
Edetate solution preparation for laboratory use.
1.0 OBJECTIVE:
To
lay down the procedure of Copper
Edetate solution for Laboratory use.
2.0 SCOPE:
This
shall be applicable to the Quality Control Laboratory.
3.0 RESPONSIBILITY:
3.1
Q.C Analyst.
4.0 ACCOUNTABILITY:
4.1
Manager-Quality
Control.
4.2
Head of the Q.C
Department.
5.0 MATERIAL AND
EQUIPMENT:
5.1
Glassware (3 beaker,
1 pipette, 1 spatula, 1 glass rod).
5.2
Magnetic stirrer.
5.3
Analytical
weighing balance.
5.4
2.0ml of 2g/l
solution of copper acetate R.
5.5
0.02g of copper
acetate.
5.6
2.0ml of 0.1M
sodium edetate.
5.7
Purified water
(q.s.).
6.0 PROCEDURE:
6.1
Firstly weigh 0.02g
of copper acetate by using analytical weighing balance operate according to
SOP.
6.2
Prepare 0.02g/10ml
solution of copper acetate R separately in beaker (i.e. equivalent to 20g/l
solution of copper acetate).
6.3
In another beaker
prepare 0.1M sodium edetate.
6.4
Take 50.0ml of
beaker and mix 2.0ml of a 0.02g/10ml solution of copper acetate R add 2.0ml of
0.1M sodium edetate.
6.5
Mix it by using
magnetic stirrer.
6.6
And dilute it to 50.0ml
with purified water R.
6.7
Again mix it
thoroughly by using magnetic stirrer.
6.8
Ready for use.
7.0 REVISION LOG:
|
Revision No.
|
Effective Date
|
Reason
|
|
00
|
|
New
SOP
|
8.0 REFERENCES:
8.1 The European Pharmacopoeia. Vol-1. Official Monograph /Reagents: 2015,
pp.425-536.
9.0 ANNEXURES:
9.1
Not Applicable.
10.0
ABBREVIATIONS:
|
Abbreviation
|
Expanded Form
|
|
SOP
|
Standard operating
procedure
|
|
&
|
And
|
|
No.
|
Number
|
|
Ltd.
|
Limited
|
|
QCR
|
Quality control reagent
|
|
Q.C
|
Quality control
|
|
ml
|
Milliliter
|
|
Vol
|
Volume
|
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