Chloroform, acidified Reagent Preparation SOP

Title: Standard operating procedure of Chloroform, acidified preparation for laboratory use.


1.0  OBJECTIVE:
To lay down the procedure of Chloroform, acidified for Laboratory use.
2.0  SCOPE:
This shall be applicable to the Quality Control Laboratory.
3.0  RESPONSIBILITY:
3.1  Q.C Analyst.
4.0  ACCOUNTABILITY:
4.1  Manager-Quality Control.
4.2  Head of the Q.C Department.
5.0  MATERIAL AND EQUIPMENT:
5.1  Glassware (1 separating funnel, 1 graduated measuring cylinder, 1 pipette).
5.2  100.0ml of chloroform.
5.3  10.0ml of hydrochloric acid.
6.0  PROCEDURE:
6.1  Take a separating funnel and add 100.0ml of chloroform and 10.0ml of hydrochloric acid.
6.2  Shake, allow it to stand and separate the 2 layers i.e. chloroform layer and acidic layer.
7.0  REVISION LOG:
Revision No.
Effective Date
Reason
00

New SOP

8.0  REFERENCES:
8.1  The European Pharmacopoeia. Vol-1. Official Monograph /Reagents: 2015, pp.425-536.
9.0  ANNEXURES:
9.1  Not Applicable.
10.0    ABBREVIATIONS:
Abbreviation
Expanded Form
SOP
Standard operating procedure
&
And
No.
Number  
Ltd.
Limited
QCR
Quality control reagent
Q.C
Quality control
ml
Milliliter
Vol
Volume


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