1.0 OBJECTIVE:
To
lay down the procedure of Chloroform,
acidified for Laboratory use.
2.0 SCOPE:
This
shall be applicable to the Quality Control Laboratory.
3.0 RESPONSIBILITY:
3.1
Q.C Analyst.
4.0 ACCOUNTABILITY:
4.1
Manager-Quality
Control.
4.2
Head of the Q.C
Department.
5.0 MATERIAL AND
EQUIPMENT:
5.1
Glassware (1
separating funnel, 1 graduated measuring cylinder, 1 pipette).
5.2
100.0ml of
chloroform.
5.3
10.0ml of
hydrochloric acid.
6.0 PROCEDURE:
6.1
Take a separating
funnel and add 100.0ml of chloroform and 10.0ml of hydrochloric acid.
6.2
Shake, allow it to
stand and separate the 2 layers i.e. chloroform layer and acidic layer.
7.0 REVISION LOG:
Revision No.
|
Effective Date
|
Reason
|
00
|
|
New
SOP
|
8.0 REFERENCES:
8.1 The European Pharmacopoeia. Vol-1. Official Monograph /Reagents: 2015,
pp.425-536.
9.0 ANNEXURES:
9.1
Not Applicable.
10.0
ABBREVIATIONS:
Abbreviation
|
Expanded Form
|
SOP
|
Standard operating
procedure
|
&
|
And
|
No.
|
Number
|
Ltd.
|
Limited
|
QCR
|
Quality control reagent
|
Q.C
|
Quality control
|
ml
|
Milliliter
|
Vol
|
Volume
|
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