1.0 OBJECTIVE:
To
lay down the operating procedure of Centrifuge machine.
2.0 SCOPE:
This
shall be applicable to the Centrifuge
machine of Quality Control Instrument Laboratory.
3.0 RESPONSIBILITY:
3.1
Technical Assistant/
Helper.
3.2
Q.C Analyst.
4.0 ACCOUNTABILITY:
4.1
Manager-Quality
Control.
5.0 MATERIAL AND
EQUIPMENT:
5.1
Centrifuge machine (Combi-Spin FVL-2400N).
6.0 PROCEDURE:
6.1
Operating
instructions:
6.1.1
Connect unit to a
properly grounded power socket.
6.1.2
Operation
in the quick centrifugation mode:
6.1.2.1
Turn the switch
into position I (QUICK SPIN).
6.1.2.2
Fill the
centrifuge tubes, all have equal volumes, with the sample solution.
6.1.2.3
Open the lid and
place EVEN number of tubes in rotor sockets facing one another.
6.1.2.4
Close the lid.
6.1.2.5
Press Quick Spin
button for quick mixing/ sedimentation and hold it pressed for the desired
time. Combi-Spin stops automatically when the Quick Spin button is released.
6.1.2.6
Turn the switch
into position O (OFF).
6.1.3
Operation
in the vortex mode:
6.1.3.1
Turn the switch
into position I (QUICK SPIN).
6.1.3.2
Fill the
centrifuge tubes, all have equal volumes, with the sample solution.
6.1.3.3
Gently holding a
tube with two fingers at its upper part dip the tube’s lower part into the
conic deepening of the vortex head.
6.1.3.4
Press Quick Spin
button for mixing and hold it pressed till full sediment solution.
6.1.3.5
Turn the switch
into position O (OFF).
6.1.4
Operation
in the long centrifugation mode:
6.1.4.1
Open the lid and
place EVEN number of tubes in rotor sockets opposite one another.
6.1.4.2
Close the lid.
6.1.4.3
Turn the switch
into position II to start centrifugation.
6.1.4.4
Turn the switch
into position O (OFF) to stop centrifugation.
6.1.5
After the
centrifuge machine stops, remove the centrifuge tubes and place in the stand
for observations
6.1.6
Disconnect unit
from the mains.
6.2
Precautions:
6.2.1
Combi-Spin will
not operate at any moment when the lid is opened.
6.2.2
Do not open the
lid during work, run-up or shut off of the rotor. The unit stops automatically
when the lid is open.
6.2.3
Centrifuge tubes
should be places in opposite side in order to balance.
6.2.4
Lid should place
over it.
6.2.5
Transfer
centrifuge tube without disturbing settled layer.
6.2.6
Always set
regulator to position O when you have to stop revolutions. Do not “OFF” it from
main switch directly.
6.2.7
If your sample is
filled only in one test tube, balance your instrument by placing a centrifuge
tube filled with water, in opposite side.
6.2.8
Switch “OFF” and
replace the plug from socket, when not in use.
7.0 REVISION LOG:
|
Revision No.
|
Effective Date
|
Reason
|
|
00
|
|
New
SOP
|
8.0 REFERENCES:
8.1
Not Applicable.
9.0 ANNEXURES:
Annexure 1: Arrangement
of centrifuge tube.
Annexure: 1
ARRANGEMENT OF CENTRIFUGE
TUBE
|
Arrangement
of centrifuge tube
 |
10.0
ABBREVIATIONS:
|
Abbreviation
|
Expanded Form
|
|
SOP
|
Standard operating
procedure
|
|
&
|
And
|
|
No.
|
Number
|
|
Ltd.
|
Limited
|
|
Q.C
|
Quality control
|
|
QCEO
|
Quality control
equipment operation
|
|
F
|
Format
|
No comments:
Post a Comment