1.0 OBJECTIVE:
To
lay down the procedure of Bromothymol
blue solution R3 for Laboratory use.
2.0 SCOPE:
This
shall be applicable to the Quality Control Laboratory.
3.0 RESPONSIBILITY:
3.1
Q.C Analyst.
4.0 ACCOUNTABILITY:
4.1
Manager-Quality
Control.
4.2
Head of the Q.C
Department.
5.0 MATERIAL AND
EQUIPMENT:
5.1
Glassware (500.0ml
beaker, 20.0ml beaker, 1 stirrer, 10.0ml pipette).
5.2
Analytical
weighing balance.
5.3
Magnetic stirrer.
5.4
Water bath.
5.5
0.1g of
bromothymol blue.
5.6
3.2ml of 0.05M
sodium hydroxide.
5.7
Alcohol (90% v/v).
5.8
0.01g of Sodium
hydroxide.
6.0 PROCEDURE:
6.1
Firstly weigh 0.1g
of bromothymol blue, 0.01g of sodium hydroxide by using analytical weighing
balance according to SOP.
6.2
Prepare 0.05M of sodium
hydroxide approximately 5.0ml. Take a beaker of 20.0ml add 5.0ml of purified
water in it and 0.01g of sodium hydroxide. Dissolve it completely.
6.3
Now take a beaker
of 500.0ml and warm 0.1g of bromothymol blue with 3.2ml of 0.05M sodium
hydroxide and 5.0ml of alcohol (90% v/v) by using water bath.
6.4
After solution is
affected, dilute it to 250.0ml with alcohol (90% v/v).
7.0 REVISION LOG:
|
Revision No.
|
Effective Date
|
Reason
|
|
00
|
|
New
SOP
|
8.0 REFERENCES:
8.1 The European Pharmacopoeia. Vol-1. Official Monograph /Reagents: 2015,
pp.425-536.
9.0 ANNEXURES:
9.1
Not Applicable.
10.0
ABBREVIATIONS:
|
Abbreviation
|
Expanded Form
|
|
SOP
|
Standard operating
procedure
|
|
&
|
And
|
|
No.
|
Number
|
|
Ltd.
|
Limited
|
|
QCR
|
Quality control reagent
|
|
Q.C
|
Quality control
|
|
R
|
Reagent
|
|
ml
|
Milliliter
|
|
g
|
Grams
|
|
mg
|
Milligram
|
|
%
|
Percentage
|
|
v/v
|
Volume by volume
|
|
M
|
Molar
|
|
q.s
|
Quantity sufficient
|
|
Vol
|
Volume
|
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