Bromothymol blue solution R3 Reagent Preparation SOP

Title: Standard operating procedure of Bromothymol blue solution R3 preparation for laboratory use.


1.0  OBJECTIVE:
To lay down the procedure of Bromothymol blue solution R3 for Laboratory use.
2.0  SCOPE:
This shall be applicable to the Quality Control Laboratory.
3.0  RESPONSIBILITY:
3.1  Q.C Analyst.
4.0  ACCOUNTABILITY:
4.1  Manager-Quality Control.
4.2  Head of the Q.C Department.
5.0  MATERIAL AND EQUIPMENT:
5.1  Glassware (500.0ml beaker, 20.0ml beaker, 1 stirrer, 10.0ml pipette).
5.2  Analytical weighing balance.
5.3  Magnetic stirrer.
5.4  Water bath.
5.5  0.1g of bromothymol blue.
5.6  3.2ml of 0.05M sodium hydroxide.
5.7  Alcohol (90% v/v).
5.8  0.01g of Sodium hydroxide.
6.0  PROCEDURE:
6.1  Firstly weigh 0.1g of bromothymol blue, 0.01g of sodium hydroxide by using analytical weighing balance according to SOP.
6.2  Prepare 0.05M of sodium hydroxide approximately 5.0ml. Take a beaker of 20.0ml add 5.0ml of purified water in it and 0.01g of sodium hydroxide. Dissolve it completely.
6.3  Now take a beaker of 500.0ml and warm 0.1g of bromothymol blue with 3.2ml of 0.05M sodium hydroxide and 5.0ml of alcohol (90% v/v) by using water bath.
6.4  After solution is affected, dilute it to 250.0ml with alcohol (90% v/v).
7.0  REVISION LOG:
Revision No.
Effective Date
Reason
00

New SOP

8.0  REFERENCES:
8.1  The European Pharmacopoeia. Vol-1. Official Monograph /Reagents: 2015, pp.425-536.
9.0  ANNEXURES:
9.1  Not Applicable.
10.0    ABBREVIATIONS:
Abbreviation
Expanded Form
SOP
Standard operating procedure
&
And
No.
Number  
Ltd.
Limited
QCR
Quality control reagent
Q.C
Quality control
R
Reagent
ml
Milliliter
g
Grams
mg
Milligram
%
Percentage
v/v
Volume by volume
M
Molar
q.s
Quantity sufficient
Vol
Volume

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