Title: Standard operating procedure of Bromothymol
blue solution R1 preparation for laboratory use.
1.0 OBJECTIVE:
To
lay down the procedure of Bromothymol
blue solution R1 for Laboratory use.
2.0 SCOPE:
This
shall be applicable to the Quality Control Laboratory.
3.0 RESPONSIBILITY:
3.1
Q.C Analyst.
4.0 ACCOUNTABILITY:
4.1
Manager-Quality
Control.
4.2
Head of the Q.C
Department.
5.0 MATERIAL AND
EQUIPMENT:
5.1
Glassware (100.0ml
beaker, 20.0ml beaker, 1 stirrer, 10.0ml pipette).
5.2
Analytical
weighing balance.
5.3
50.0mg of
bromothymol blue (approx. 0.05g).
5.4
4.0ml of 0.02M
sodium hydroxide.
5.5
20.0ml of alcohol.
5.6
0.004g of Sodium
hydroxide.
5.7
Purified water
(q.s).
6.0 PROCEDURE:
6.1
Firstly weigh
50.0mg of bromothymol blue, 0.004g of sodium hydroxide by using analytical
weighing balance according to SOP.
6.2
Prepare 0.02M of
sodium hydroxide approximately 5.0ml. Take a beaker of 20.0ml add 5.0ml of
purified water in it and 0.004g of sodium hydroxide. Dissolve it completely.
6.3
Now take a beaker
of 100.0ml and dissolve 50.0mg of bromothymol blue in a mixture of 4.0ml of
0.02M sodium hydroxide and 20.0ml of alcohol by using stirrer.
6.4
Dilute it to
100.0ml with purified water.
7.0 REVISION LOG:
Revision No.
|
Effective Date
|
Reason
|
00
|
|
New
SOP
|
8.0 REFERENCES:
8.1 The European Pharmacopoeia. Vol-1. Official Monograph /Reagents: 2015,
pp.425-536.
9.0 ANNEXURES:
9.1
Not Applicable.
10.0
ABBREVIATIONS:
Abbreviation
|
Expanded Form
|
SOP
|
Standard operating
procedure
|
&
|
And
|
No.
|
Number
|
Ltd.
|
Limited
|
QCR
|
Quality control reagent
|
Q.C
|
Quality control
|
R
|
Reagent
|
ml
|
Milliliter
|
g
|
Grams
|
mg
|
Milligram
|
M
|
Molar
|
q.s
|
Quantity sufficient
|
Vol
|
Volume
|
No comments:
Post a Comment