Bromothymol blue solution R1 Reagent Preparation SOP


Title: Standard operating procedure of Bromothymol blue solution R1 preparation for laboratory use.
1.0  OBJECTIVE:
To lay down the procedure of Bromothymol blue solution R1 for Laboratory use.
2.0  SCOPE:
This shall be applicable to the Quality Control Laboratory.
3.0  RESPONSIBILITY:
3.1  Q.C Analyst.
4.0  ACCOUNTABILITY:
4.1  Manager-Quality Control.
4.2  Head of the Q.C Department.
5.0  MATERIAL AND EQUIPMENT:
5.1  Glassware (100.0ml beaker, 20.0ml beaker, 1 stirrer, 10.0ml pipette).
5.2  Analytical weighing balance.
5.3  50.0mg of bromothymol blue (approx. 0.05g).
5.4  4.0ml of 0.02M sodium hydroxide.
5.5  20.0ml of alcohol.
5.6  0.004g of Sodium hydroxide.
5.7  Purified water (q.s).
6.0  PROCEDURE:
6.1  Firstly weigh 50.0mg of bromothymol blue, 0.004g of sodium hydroxide by using analytical weighing balance according to SOP.
6.2  Prepare 0.02M of sodium hydroxide approximately 5.0ml. Take a beaker of 20.0ml add 5.0ml of purified water in it and 0.004g of sodium hydroxide. Dissolve it completely.
6.3  Now take a beaker of 100.0ml and dissolve 50.0mg of bromothymol blue in a mixture of 4.0ml of 0.02M sodium hydroxide and 20.0ml of alcohol by using stirrer.
6.4  Dilute it to 100.0ml with purified water.
7.0  REVISION LOG:
Revision No.
Effective Date
Reason
00

New SOP

8.0  REFERENCES:
8.1  The European Pharmacopoeia. Vol-1. Official Monograph /Reagents: 2015, pp.425-536.
9.0  ANNEXURES:
9.1  Not Applicable.
10.0    ABBREVIATIONS:
Abbreviation
Expanded Form
SOP
Standard operating procedure
&
And
No.
Number  
Ltd.
Limited
QCR
Quality control reagent
Q.C
Quality control
R
Reagent
ml
Milliliter
g
Grams
mg
Milligram
M
Molar
q.s
Quantity sufficient
Vol
Volume


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