Bromophenol blue solution Reagent Preparation SOP

Title: Standard operating procedure of Bromophenol blue solution preparation for laboratory use

1.0  OBJECTIVE:

To lay down the procedure of Bromophenol blue solution for Laboratory use.

2.0  SCOPE:

This shall be applicable to the Quality Control Laboratory.

3.0  RESPONSIBILITY:

3.1  Q.C Analyst.

4.0  ACCOUNTABILITY:

4.1  Manager-Quality Control.

4.2  Head of the Q.C Department.

5.0  MATERIAL AND EQUIPMENT:

5.1  Glassware (100.0ml beaker, stirrer, 10.0ml pipette).

5.2  Analytical weighing balance.

5.3  0.1g of bromophenol blue.

5.4  1.5ml of 0.1M sodium hydroxide.

5.5  20.0ml of alcohol (ethanol).

5.6  Purified water (q.s).

6.0  PROCEDURE:

6.1  Firstly weigh 0.1g of bromophenol blue, 0.028g of sodium hydroxide by using analytical weighing balance according to SOP.

6.2  Prepare 0.1M of sodium hydroxide solution (prepare 5.0ml according to need).

Take a beaker of 50.0ml and add 0.028g of sodium hydroxide in it and dissolve it in 5.0ml of purified water to make 0.1M of sodium hydroxide solution.

6.3  Take a beaker of 100.0ml and add 0.1g of bromophenol blue in it.

6.4  Also add 1.5ml of 0.1M sodium hydroxide and 20.0ml of alcohol by using pipette.

6.5  Dissolve it thoroughly.

6.6  And dilute it to 100.0ml of purified water.

7.0  REVISION LOG:

Revision No.	Effective Date	Reason
00		New SOP

8.0  REFERENCES:

8.1  The European Pharmacopoeia. Vol-1. Official Monograph /Reagents: 2015, pp.425-536.

9.0  ANNEXURES:

9.1  Not Applicable.

10.0    ABBREVIATIONS:

Abbreviation	Expanded Form
SOP	Standard operating procedure
&	And
No.	Number
Ltd.	Limited
QCR	Quality control reagent
Q.C	Quality control
ml	Milliliter
g	Grams
M	Molar
mg	Milligram
q.s	Quantity sufficient
Vol	Volume