Title: Standard operating procedure of Ascorbic
acid solution for laboratory use.
1.0 OBJECTIVE:
To
lay down the procedure of Ascorbic
acid solution for Laboratory use.
2.0 SCOPE:
This
shall be applicable to the Quality Control Laboratory.
3.0 RESPONSIBILITY:
3.1
Q.C Analyst.
4.0 ACCOUNTABILITY:
4.1
Manager-Quality
Control.
4.2
Head of the Q.C
Department.
5.0 MATERIAL AND
EQUIPMENT:
5.1
Glassware (one 50.0ml
beaker, one stirrer, one 10.0ml pipette).
5.2
Analytical
weighing balance.
5.3
50.0mg of Ascorbic acid.
5.4
0.5ml of Purified
water.
5.5
Dimethylformamide
(q.s).
6.0 PROCEDURE:
6.1
Take a beaker of 50.0ml.
6.2
Weigh 50.0mg of
ascorbic acid, operate analytical weighing balance according to SOP.
(50.0mg=0.05g)
6.3
Add 50.0mg of the
ascorbic acid in beaker and add 0.5ml of purified water with the help of
pipette.
6.4
Dissolve it by
using stirrer.
6.5
And dilute it to
50.0ml with Dimethylformamide.
7.0 REVISION LOG:
|
Revision No.
|
Effective Date
|
Reason
|
|
00
|
|
New
SOP
|
8.0 REFERENCES:
8.1 The British Pharmacopoeia. Vol V. Appendix I, Official Monograph /General Reagent: 2015,
pp.19-141.
9.0 ANNEXURES:
9.1
Not Applicable.
10.0
ABBREVIATIONS:
|
Abbreviation
|
Expanded Form
|
|
SOP
|
Standard operating
procedure
|
|
&
|
And
|
|
No.
|
Number
|
|
Ltd.
|
Limited
|
|
Q.C
|
Quality control
|
|
ml
|
Milliliter
|
|
mg
|
Milligram
|
|
g
|
Grams
|
|
q.s
|
Quantity sufficient
|
|
Vol
|
Volume
|
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