Ascorbic acid Reagent Preparation SOP

Title: Standard operating procedure of Ascorbic acid solution for laboratory use.

1.0  OBJECTIVE:

To lay down the procedure of Ascorbic acid solution for Laboratory use.

2.0  SCOPE:

This shall be applicable to the Quality Control Laboratory.

3.0  RESPONSIBILITY:

3.1  Q.C Analyst.

4.0  ACCOUNTABILITY:

4.1  Manager-Quality Control.

4.2  Head of the Q.C Department.

5.0  MATERIAL AND EQUIPMENT:

5.1  Glassware (one 50.0ml beaker, one stirrer, one 10.0ml pipette).

5.2  Analytical weighing balance.

5.3  50.0mg of Ascorbic acid.

5.4  0.5ml of Purified water.

5.5  Dimethylformamide (q.s).

6.0  PROCEDURE:

6.1  Take a beaker of 50.0ml.

6.2  Weigh 50.0mg of ascorbic acid, operate analytical weighing balance according to SOP. (50.0mg=0.05g)

6.3  Add 50.0mg of the ascorbic acid in beaker and add 0.5ml of purified water with the help of pipette.

6.4  Dissolve it by using stirrer.

6.5  And dilute it to 50.0ml with Dimethylformamide.

7.0  REVISION LOG:

Revision No.	Effective Date	Reason
00		New SOP

8.0  REFERENCES:

8.1  The British Pharmacopoeia. Vol V. Appendix I, Official Monograph /General Reagent: 2015, pp.19-141.

9.0  ANNEXURES:

9.1  Not Applicable.

10.0    ABBREVIATIONS:

Abbreviation	Expanded Form
SOP	Standard operating procedure
&	And
No.	Number
Ltd.	Limited
Q.C	Quality control
ml	Milliliter
mg	Milligram
g	Grams
q.s	Quantity sufficient
Vol	Volume