Ammonium Molybdate Reagent R1 Preparation SOP

Title:Standard operating procedure of Ammonium molybdate reagent R1 preparation for laboratory use.

1.0  OBJECTIVE:
To lay down the procedure of Ammonium molybdate reagent R1 for Laboratory use.
2.0  SCOPE:
This shall be applicable to the Quality Control Laboratory.
3.0  RESPONSIBILITY:
3.1  Q.C Analyst.
4.0  ACCOUNTABILITY:
4.1  Manager-Quality Control.
4.2  Head of the Q.C Department.
5.0  MATERIAL AND EQUIPMENT:
5.1  Glassware (one 500.0ml beaker, one stirrer, one 10.0ml pipette).
5.2  Analytical weighing balance.
5.3  Magnetic stirrer.
5.4  0.6g of disodium arsenate.
5.5  10ml of 0.6g/10ml of disodium arsenate solution.
5.6  50.0ml ammonium molybdate solution.
5.7  90.0ml of dilute sulphuric acid.
5.8  Purified water (q.s).
6.0  PROCEDURE:
6.1  Firstly weigh 0.6g of disodium arsenate by using analytical weighing balance operate according to SOP.
6.2  Prepare 0.6g/10ml solution of disodium arsenate solution separately in beaker.
6.3  Take 500.0ml of beaker and mix 10.0ml of 0.6g/10ml solution of disodium arsenate, 50.0ml of ammonium molybdate solution, 90.0ml of dilute sulphuric acid.
6.4  Mix it by using magnetic stirrer.
6.5  And dilute it to 200.0ml with purified water.
6.6  Again mix it thoroughly by using magnetic stirrer.
6.7  Ready for use.
7.0  REVISION LOG:
Revision No.
Effective Date
Reason
00

New SOP

8.0  REFERENCES:
8.1  The European Pharmacopoeia. Vol-1. Official Monograph /Reagents: 2015, pp.425-536.
9.0  ANNEXURES:
9.1  Not Applicable.
  
10.0    ABBREVIATIONS:
Abbreviation
Expanded Form
SOP
Standard operating procedure
&
And
No.
Number
Ltd.
Limited
Q.C
Quality control
ml
Milliliter
g
Grams
q.s
Quantity sufficient
Vol
Volume
R
Reagent

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