Preparation of
Disinfectant Solution
1.
Purpose:-
This procedure is developed to ensure correct and
safe preparation of disinfectant solution.
2.
Scope:-
This Procedure applies to cleaning of aseptic area.
3.
HSE
Statement:-
3.1.
Follow the following instructions to perform the
subjected job in safe manners.
3.1.1.
Only trained and knowledgeable persons perform subjected
job.
3.1.2.
All work is performed in accordance with applicable
regulatory requirements.
3.1.3.
Report any job related injuries or illnesses, questions
on health and safety, or any unsafe or unhealthy working conditions to
supervisor.
3.1.4.
Personal protective equipment necessary to perform subjected
job in safe manners.
4.
Responsibilities:-
4.1.
Lab Assistant
is responsible to conduct assigned tasks in a safe manner and report to
supervisor in case of any problem.
4.2.
Lab. Assistant
is responsible to carry out the preparation of disinfectant solution under his
supervision.
4.3.
Microbiologist
is responsible to manage all activities include operator training, record
keeping, proper use of personal protective equipment & other related tasks.
5.
Materials:-
i.
Measuring cylinder
ii.
Beaker
iii.
Disinfectant chemical
iv.
Spray bottles
v.
SS buckets
6.
Definition:-
6.1. Disinfectant Solution
The solution used to clear the area from viable
micro-organisms (microbes)
7.
Flow Chart:-
N/A
8.
Description:-
8.1. Procedure:-
ü
Clean all utensils thoroughly with Filtered
W.F.I (70 – 80 oC).
ü
Use the sterilized buckets for preparation of
solution.
ü
Fill the spray bottles and buckets with
disinfectant solution (as per composition below) and keep them at designated
locations.
8.2. List of Disinfectant With Composition
8.2.1.
Mopping Solution
5 % Phenol
Composition for 5.0 L
Phenol (100 %) = 250 ml
W.F.I (QS) = 5000 ml
0.6%Proceine
40(A Synergistic mixture of Alkyl-Amino-Alkyl-Glycine)
Composition for 5.0
L
Proceine 40 = 30 ml
W.F.I (QS) = 5.0 lit.
2%Vanoquat
(Quaternary Ammonium Compound)
Composition for 5.0 L
Vanoquat = 100 ml
W.F.I (QS) = 5000 ml
8.2.2.
Solutions for
Gloved Hands
70 % IPA
Composition for 100 ml
IPA (95 %) = 74 ml
W.F.I(QS) = 100 ml
8.2.3.
For Fumigation:
25% Biocide
B (Chlorine Dioxide + Quaternary
Ammonium Compound)
Composition for 500 ml
Biocide B = 125ml (1 Part)
W.F.I (QS) = 500ml (3Part)
2%
Glutaraldehyde *
Composition for 500 ml
Glutaraldehyde = 10ml
W.F.I (QS) = 500 ml
8.3. Precautions:-
8.3.1.
Prepare daily fresh disinfectant solution for cleaning
and mopping.
8.3.2.
Prepare disinfectant solution in aseptic area.
8.3.3.
Use Sterilized W.F.I. for dilutions.
8.3.4.
Measure exact quantities of disinfectant and
W.F.I.
8.3.5.
Rotate the Disinfectant on weekly Basis.
8.3.6.
Maintain the Preparation & Rotation record.
9.
Record:-
9.1.
RG-04-07 Preparation
& Rotation Record of Disinfectant Solution
10. Reference:-
10.1. *
USP 36 Edition Volume I
11. Distribution:-
|
Sr. #
|
Distributed
to
|
Received
(Current)
|
Returned
(Obsolete)
|
|
01
|
Quality Control Department
|
|
|
|
02
|
Quality Assurance Department
|
|
|
|
03
|
Quality Management Department
|
|
|
12. Revision History:-
Date Changes
N/A