Operation of Hot Air Oven
1. Purpose:-
It is established to provide a Procedure for drying
and depyrogenation of Lab glassware & small equipment.
2. Scope:-
It is applicable to the oven in Quality Control Laboratory.
3. HSE Statement:-
Not Required.
4. Responsibilities:-
i)
Manager Quality Control is responsible to ensure
that procedure & formats are followed entirely as approved.
ii)
Microbiologist is responsible to operate the instrument
5. Materials:-
N/A
6. Definitions:-
6.1
Sterilization:-is a term referring to any process that
eliminates (removes) or kills all forms of
microbial life, including transmissible
agents (such as fungi,
bacteria, viruses,
spore forms, etc.)
present on a surface, contained in a fluid, in medication, or in a
compound such as biological
culture media.
Sterilization can be achieved by applying the .proper combinations of heat,
6.2 Dry heat sterilization of an article is one of the earliest
forms of sterilization practiced. Dry heat, as the name indicates, utilizes hot
air that is either free from water vapour, or has very little of it, and where
this moisture plays a minimal or no role in the process of sterilization
8. Description:-
8.1 Procedure:-
8.1.1 Ensure that the instrument
is clean & free from dust.
8.1.2 Switch “ON” the mains.
8.1.3 The green indication light
will be “ON”
8.1.4 There
is a three – way switch for the selection of operational menu.
8.1.5 The required temperature (e.g. 105 o
C) can be set by a thermostatically controlled knob, by rotating
it in
clockwise or anti-clockwise direction. Note – When temperature is increased, a
yellow –
indication
light will be “ON” and then will be “OFF” automatically.
8.1.6 Inside
the oven, there are two trays. Put any object that required drying, on this
tray and close the
door of the oven.
8.1.7 Adjust the fresh air from slide knob.
8.1.8 For sterilizing instruments and glass apparatus cover them with aluminum
foil and then place them
in oven.
8.2 Cleaning:-
8.2.1 The surfaces of the hot air oven can
be cleaned with commercially available
cleaning agents.
8.2.2 It
is important to ensure that no rust forming object comes in to contact with
the chamber. If any
contamination
causes rust stains on the surfaces of the chamber, such spots must be cleaned
off immediately and the area should be polished.
8.2.3 The control panel, the plastic input
modules and other plastic components of the hot air oven
must
not be cleaned using scouring cleaning agents or those contained solvents.
8.2. Lubricate all moving parts of the door
using thin silicon grease and to check that the hinge screws
are tight.
8.3 Safety
Precautions:-
8.3.1 Do not place any load on the floor
against the side walls or underneath the ceiling of the chamber in
order to ensure
proper air circulation inside the chamber.
8.3.2 Place the unit away from draft,
ventilating outlets, radiators or any other device that may cause
severe
changes in the temperatures conditions surrounding the incubator cabinet.
8.3.4 Place the shelves in their proper
positions provided inside the oven chamber side before loading the
material.
Load the shelves evenly.
8.3.5 As a routine working precaution always wear safety mask and protective
gloves when operating
loading
and unloading the incubator.
8.4 Calibration
Frequency:-
Annually from external source.
9. Records:-
Daily
basis temperature record for incubators and ovens
10. References:-
Manufacturer
instructions Manual
11. Distribution:-
This SOP has to be distributed in below mentioned
Departments:-
|
Sr. NO
|
Distributed to
|
Received
(Current)
|
Returned
(Obsolete)
|
|
1
|
Quality Control
Department
|
|
|
|
2
|
Quality Management
Department
|
|
|
12.
Revision History:-
N/A