Operation of Constant Temperature Incubator
1. Purpose:-
To provide a
procedure for the operation of Constant
Temperature Incubator
2. Scope:-
It is applicable in Microbiology section of the Quality Control
Department.
3. HSE
Statement:-
Not Applicable.
4.
Responsibilities:-
i)
Manager Quality Control is responsible to ensure
that procedure & formats are followed entirely as approved.
ii)
Microbiologist is responsible to operate the
instrument.
5.
Materials:-
N/A
6. Definitions:-
6.1 Incubator:-
Incubator is a device used to grow and maintain microbiological
cultures or cell cultures. The incubator maintains
optimal temperature, humidity and other conditions such as the
carbon dioxide
(CO2) and oxygen content of the atmosphere inside.
8.
Description:
8.1 Technical Specifications:-
Make:
- Rowsen - China
Model: - PHW 165 Q
Capacity: - 165 L
Ambient temp.:- +5°C ~50°C
8.2 Procedure:-
8.2.1 Switch On the apparatus.
8.2.2 Temperature display: Display the
current temperature inside the cabinet.
8.2.3 Temperature setting key: Three-digit dial key (one decimal),
press“-” to decrease the numerical value, press “+ “to increase
the numerical value.
8.2.4
The indicator
‘ON’: Light is bright during heating.
8.2.5
The indicator
‘OFF’: Light is bright during no
heating
8.3 Cleaning:-
8.3.1 The surfaces of the Constant Temperature
Incubator can be cleaned with commercially available
cleaning
agents.
8.3.2
It is important to ensure that no rust forming object
comes in to contact with the chamber. If any contamination causes rust stains
on the surfaces of the chamber, such spots must be cleaned off immediately and
the area should be polished.
8.3.3
The control panel, the plastic input modules and other
plastic components of the Constant Temperature Incubator must not be cleaned
using scouring cleaning agents or those contained solvents.
8.3.4
Lubricate all moving parts of the door using thin
silicon grease and to check that the hinge screws are tight.
8.4 Safety
Precautions:-
8.4.1 The floor should be firm and level.
8.4.2 Do
not place any load on the floor against the side walls or underneath the
ceiling of the chamber in
order to ensure proper air circulation inside
the chamber.
8.4.3
Always place
the Constant Temperature
incubator in a well ventilated area.
8.4.4
As a routine
working precaution, always wear safety mask and protective gloves when
operating loading and unloading the Constant Temperature incubator.
8.5 Calibration Frequency:-
Annually from external source.
9. References:-
Manufacturer
instructions Manual
10.
Records:-
Daily basis temperature record
for incubators and ovens
11.
Distribution:-
This SOP has to be distributed in below mentioned
Departments:-
|
Sr. NO
|
Distributed to
|
Received
(Current)
|
Returned
(Obsolete)
|
|
1
|
Quality Control Department
|
|
|
|
2
|
Quality Management
Department
|
|
|
12. Revision History:-
N/A