Microscope Operational SOP


Microscope Operational SOP

1.0  OBJECTIVE:
To lay down the operating procedure of Microscope.
2.0  SCOPE:
This shall be applicable to the Microscope of Microbiology laboratory.
3.0  RESPONSIBILITY:
3.1  Technical Assistant/ Helper.
3.2  Microbiologist.
4.0  ACCOUNTABILITY:
4.1  Manager-Quality Control.
5.0  MATERIAL AND EQUIPMENT:
5.1  Microscope.
6.0  PROCEDURE:
6.1  Ensure that the instrument is clean according to the SOP.
6.2  Keep the microscope in position on a solid vibration free surface.
6.3  Switch on the main switch of microscope, the light bulb turns ON.
6.4  Adjust the low power objective, by rotating the revolving nose piece in position.
6.5  Adjust the focus of light with the help of mirror.
6.6  Now place the objective in position by moving the revolving nose piece.
6.7  Place the slides on the stage, lower the objective, while observing through the eye piece, till the filed appears clear (if the oil immersion objective is in use, place one drop of immersion oil on the slide before placing it on the stage and then lower the objective lens just to touch the oil drop).
6.8  Make the filed clearer with the help of fine adjustment and then observe the slide.
6.9  Slide can be moved in left, right, upper and lower direction to observe the complete smear area with help of slide stage screw and coarse adjustment.
6.10    After completion of observation, remove the slide, clean the objective lens with cotton soaked in xylene.
6.11    Switch it OFF from the mains.
6.12    Precautions:
6.12.1    Do not allow the oil to harden on the lens.
6.13    After completion of work covers the microscope with plastic bag.
7.0  REVISION LOG:
Revision No.
Effective Date
Reason
00

New SOP

8.0  REFERENCES:
8.1  Not Applicable.
9.0  ANNEXURES:
9.1  Not Applicable.
10.0    ABBREVIATIONS:
Abbreviation
Expanded Form
SOP
Standard operating procedure
&
And
No.
Number  
Ltd.
Limited
Q.C
Quality control
QCEO
Quality control equipment operation
F
Format


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