Mechanical pipettor Calibration SOP


1.0  OBJECTIVE:
To lay down the calibration procedure of Mechanical pipettor for reliable and accurate results during mixing.
2.0  SCOPE:
This shall be applicable to the Mechanical pipettor of Quality Control Instrument Laboratory.
3.0  RESPONSIBILITY:
3.1  Technical Assistant/ Helper.
3.2  Q.C Analyst.
4.0  ACCOUNTABILITY:
4.1  Manager-Quality Control.
5.0  MATERIAL AND EQUIPMENT:
5.1  Mechanical pipettor.
6.0  PROCEDURE:
6.1  Maintain the Mechanical pipettor according to the SOP
6.2  Operate the Mechanical pipettor according to the SOP
6.3  Calibration should be done every 3 month with weight variation.
6.4  Different set of volumes should be taken and linearity should be performed.
6.5  Attach the micropipette tips to micropipette. The desired volume is set and water shall be aspirated. Dispensed into dry beaker and weighed repeat for 10 times. The mean of the weights are converted into μl using the following formula:
Volume = _____Weight of water sample._____
             Density of water (at 25oC)
6.6   The percentage variation can be calculated by using following formula:
Percentage variation = Actual weight – Observed weight x 100
Actual weight
6.7   Perform the calibration by suing the set volume.
6.8  Record the result on the “Calibration Record”, “Calibration Log Book” & paste the “Calibration Status Sticker” on Mechanical pipettor.
6.9  Precautions:
6.9.1     Use the analytical balance with a readability of 0.1mg.
7.0  REVISION LOG:
Revision No.
Effective Date
Reason
00

New SOP

8.0  REFERENCES:
8.1  Not Applicable.
9.0  ANNEXURES:
Annexure 1: Calibration report of Mechanical pipettor.















Annexure: 1
Calibration report of Mechanical pipettor
Calibration report of Mechanical pipettor

Calibration date
Date of last calibration done
Next due for calibration




Instrument details
Instrument name
Instrument make
Instrument identification No.



Observation Chart
Sr. #
Volume taken in
(μl)
Tolerance limit
Remarks
Min
Max





















Limit: RSD of Net volume of sample NMT 1%.



Conclusion: Satisfactory/ Not satisfactory.

Done by/date
Checked by/date



10.0    ABBREVIATIONS:
Abbreviation
Expanded Form
SOP
Standard operating procedure
&
And
No.
Number  
Ltd.
Limited
Q.C
Quality control
Q.A
Quality assurance
QCEB
Quality control equipment calibration
F
Format


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