FTIR Spectrometer Operational SOP



1.0  OBJECTIVE:
To lay down the operating procedure of Fourier transformer infrared radiation (FTIR).
2.0  SCOPE:
This shall be applicable to the Fourier transformer infrared radiation (FTIR) of Quality Control Instrument Laboratory.
3.0  RESPONSIBILITY:
3.1  Technical Assistant/ Helper.
3.2  Q.C Analyst.
4.0  ACCOUNTABILITY:
4.1  Manager-Quality Control.
5.0  MATERIAL AND EQUIPMENT:
5.1  Fourier transformer infrared radiation (FTIR).
6.0  PROCEDURE:
6.1  “Switch on” FTIR, Computer &  printer. Place sample plate (empty ) in sampling compartment.
6.2  Double click on software with the name of ²spectrum².
6.3  Login window open,  Enter login name, if required otherwise click on ²O.K²
6.4  Spectrum window open as graph. (X-axis, Y-axis).
6.5  BACKGROUND SCANING
6.5.1        Click on “instrument” on tool bar.
6.5.2        Click on ²SCAN BACK GROUND² at first time only.
6.5.3        Scan back ground window open, enter² FILE NAME² as BKG or other Words & description as² Back ground².
6.5.4        Select “start range”,   “end range” as required for scanning.
6.5.5        Select number of scans (normally 6.4).
6.5.6        Click on ² SET UP ² in the same window.
6.5.7        New window open with the name of ²SPECTRUM BX FT- IR
6.5.8        INSTRUMENT SET UP². Enter in ²  DESCRIPTION²  if required.
6.5.9        Click on  ² SCAN PARAMETER ² in the same window.
Enter the following.
6.5.10    Click Beam type Box and Beam type window open.
6.5.11    Select in ² BACK GROUND² Then ¢O.K¢.
6.5.12    In Data type Base, always ¢Single¢.
6.5.13    Click on ²UP DATE²
6.5.14    Click on ²O.K ². It will be return on the ²SCAN BACK GROUND² Window. Click on ²O.K ² Instrument start automatically to scan back ground and open window for ² Name ² Enter name of file as required. i.e. BKG etc. and click on new name or overwrite.
6.6  SAMPLE SCANING
6.6.1        Click on instrument on tool bar. Then click on ² Scan sample²
6.6.2        Collect sample plate from instrument sampling compartment.
6.6.3        Pour sample powder /liquid on sample plate and press powder with spatula manually to ensure complete filling.
6.6.4        Put sample plate in sampling compartment carefully.
6.6.5        Put powder press bar on sample plate and push lever down and screw the press bar to press the powder uniformly and close the sampling compartment.
6.6.6        Click on² instrument ²on tool bar, then click on ² SCAN SAMPLE²
6.6.7        Scan sample window open automatically.
Enter the following
In file name box write the sample name and write in description box ²SAMPLE²  ²RATIO ² appear in window as ²SELECTED²
6.6.8        Click on ²setup² in the same window and new window appear automatically.
6.6.9        Write down in description box (if required).
6.6.10    Click on ²SCAN PARAMETER²   and new window open.
6.6.11    Click on beam type box, beam type window open.
6.6.12    Select ²SAMPLE² In this window and click ²O.K² Click on ²DATA TYPE BASE ² New window open and select On ²RATIO² Then click on ²O.K ²
6.6.13    Click on² UP DATE² again scan sample window appear, click on ² OK², Instrument automatically scan spectra and name window open. Write down the name of file and click on new file or overwrite. Both spectra  ² BACK GROUND SPECTRUM ² and ²SAMPLE SPECTRA² appear on the screen. Select ²BACK GROUND ²at bottom of the screen and press ²DELETE². Back ground spectra disappear from the screen.
6.6.14    Select the sample spectra with ² CURSOR ² and double click through mouse to optimize the spectrum.
6.6.15    Click on label peak to label the peak.
6.6.16    Click ²ABC² to add test in spectra i.e. name of the compound etc. Click print to print the spectra.
6.7  PRECAUTION:
6.7.1        Preserve instrument from moisture.
6.7.2        Instrument switch on daily Basis.
6.7.3        Always clean the instrument with dry lint free cloth.
6.7.4        Avoid placing body parts in the path of light in sampling chamber.
6.7.5        Clean the sampling plate with dry and moist tissue paper, dry it before use.
6.7.6        Change desiccators after each six month and purge with nitrogen gas.
7.0  REVISION LOG:
Revision No.
Effective Date
Reason
00

New SOP
8.0  REFERENCES:
8.1  Not Applicable.
9.0  ANNEXURES:
Annexure 1: FTIR Usage Logbook.
Annexure: 1
FTIR Usage Logbook
FTIR USAGE LOGBOOK
Sr. #
Date
Product/ material name
Batch no./ Lot no.
Time
Analyzed by
Remarks
Started at
Completed on



































































































10.0    ABBREVIATIONS:
Abbreviation
Expanded Form
SOP
Standard operating procedure
&
And
No.
Number  
Ltd.
Limited
Q.C
Quality control
QCEO
Quality control equipment operation
F
Format
KBr
Potassium bromide
FTIR
Fourier transforms infrared radiation
cm-1
Per centimeter
DRA



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