FTIR Spectrometer Calibration SOP



1.0  OBJECTIVE:
To lay down the calibration procedure of Fourier transforms infrared radiation (FTIR).
2.0  SCOPE:
This shall be applicable to the Fourier transforms infrared radiation (FTIR) of Quality Control Laboratory.
3.0  RESPONSIBILITY:
3.1  Technical Assistant/ Helper.
3.2  Q.C Analyst.
4.0  ACCOUNTABILITY:
4.1  Manager-Quality Control.
5.0  MATERIAL AND EQUIPMENT:
5.1  Fourier transforms infrared radiation (FTIR).
6.0  PROCEDURE:
6.1  Switch on the system.
6.2  Set instrument parameter as follow:
6.2.1        RESOLUTION: 2.0.
6.2.2        APODIZATION STRONG
6.2.3        RANGE: 4000-400cm-1.
6.2.4        MODE RATIO.
6.2.5        NUMBER OF SCAN.
6.3  Allow the instrument to warm for 39 minutes.
6.4  Operate the instrument as per operation SOP.
6.5  Open the sample compartment cover of FTIR 1600 and place the polystyrene film in the sample holder and close the cover.
6.6  Click OK in the SCAN MODE.
6.7  The spectrum of the polystyrene film is displays on the screen.
6.8  PRINT the spectrum by going to print function.
6.9  When the printer of the spectra is completely go to DATA and then to PEAK.
6.10                            The peak data’s of the spectra of the polystyrene film is displays on the screen.
6.11                            PRINT out these data’s by going to print function.
6.12                            Compare the wave function accuracy with the following limit.
Limit of wave number accuracy
Wave number
Limit
3060.0
(±1.5)
2849.5
(±1.5)
1942.9
(±1.5)
1601.2
(±1.5)
1583.0
(±1.5)
1154.5
(±1.5)
1026.3
(±1.5)
Resolution performance of the apparatus
Record the spectrum of the polystyrene film 0.04mm in thickness. The difference x between the percentage transmittance at the transmission maximum A at 2870cm-1 (3.48μm) and that at the transmission minimum B at 2849.5cm-1 (3.51μm) should greater than 16. The difference y between the percentage transmittance at the transmission maximum C at 1589cm-1 (5.29μm) and that at the transmission minimum D at 1583cm-1 (5.32μm) should be greater than 12.
6.13                            Record the observation in the calibration record i.e. annexure-2.
6.14                            Affix a “CALIBRATION LABEL” on the instrument.
6.15                            Report any discrepancy observed during calibration or operation of the instrument to QA Manager and notify the defect to engineering dept./service. Affix an “UNDER MAINTENANCE”; label on the instrument.
6.16                            Frequency of calibration:
6.16.1    Once in three month and after each maintenance job.


7.0  REVISION LOG:
Revision No.
Effective Date
Reason
00

New SOP

8.0  REFERENCES:
8.1  Not Applicable.
9.0  ANNEXURES:
Annexure 1: Calibration record of Fourier Transforms Infrared Radiation (FTIR).




















Annexure: 1
Calibration record of Fourier Transforms Infrared Radiation (FTIR)
CALIBRATION RECORD OF FOURIER TRANSFORMS INFRARED RADIATION
Calibration date
Date of last calibration done
Next due for calibration



INSTRUMENT DETAILS:
Name of equipment:
Fourier transforms infrared radiation (FTIR)
Equipment serial no.:

Make and model:

Frequency:

OBSERVATIONS
Limit of wave number accuracy by polystyrene film:
Sr.#
Wave number
Observation
Limit
Remarks
1.       
3060.0(±1.5)

(±1.5)

2.       
2849.5(±1.5)

(±1.5)

3.       
1942.9(±1.5)

(±1.5)

4.       
1601.2(±1.0)

(±1.0)

5.       
1583.0(±1.0)

(±1.0)

6.       
1154.5(±1.0)

(±1.0)






Resolution performance by polystyrene film:
Sr.#
Wave number
Absorbance
Transmittance difference between x and y
Limit greater than in %
Remarks
1.       
2870.0




2.       
2849.5




3.       
1589.0




4.       
1583.0

















Remarks: The instrument is working satisfactorily/ not satisfactorily as per SOP.

Calibrated by/date
Checked by/date






10.0    ABBREVIATIONS:
Abbreviation
Expanded Form
SOP
Standard operating procedure
&
And
No.
Number  
Ltd.
Limited
Q.C
Quality control
Q.A
Quality assurance
QCEB
Quality control equipment calibration
F
Format
FTIR
Fourier transforms infrared radiation
cm-1
Per centimeter
mm
Millimeter
μm
Micrometer
Dept.
Department
%
Percentage


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