Centrifuge Machine Operational SOP



1.0  OBJECTIVE:
To lay down the operating procedure of Centrifuge machine.
2.0  SCOPE:
This shall be applicable to the Centrifuge machine of Quality Control Instrument Laboratory.
3.0  RESPONSIBILITY:
3.1  Technical Assistant/ Helper.
3.2  Q.C Analyst.
4.0  ACCOUNTABILITY:
4.1  Manager-Quality Control.
5.0  MATERIAL AND EQUIPMENT:
5.1  Centrifuge machine (Combi-Spin FVL-2400N).
6.0  PROCEDURE:
6.1  Operating instructions:
6.1.1        Connect unit to a properly grounded power socket.
6.1.2        Operation in the quick centrifugation mode:
6.1.2.1  Turn the switch into position I (QUICK SPIN).
6.1.2.2  Fill the centrifuge tubes, all have equal volumes, with the sample solution.
6.1.2.3  Open the lid and place EVEN number of tubes in rotor sockets facing one another.
6.1.2.4  Close the lid.
6.1.2.5  Press Quick Spin button for quick mixing/ sedimentation and hold it pressed for the desired time. Combi-Spin stops automatically when the Quick Spin button is released.
6.1.2.6  Turn the switch into position O (OFF).
6.1.3        Operation in the vortex mode:
6.1.3.1  Turn the switch into position I (QUICK SPIN).
6.1.3.2  Fill the centrifuge tubes, all have equal volumes, with the sample solution.
6.1.3.3  Gently holding a tube with two fingers at its upper part dip the tube’s lower part into the conic deepening of the vortex head.
6.1.3.4  Press Quick Spin button for mixing and hold it pressed till full sediment solution.
6.1.3.5  Turn the switch into position O (OFF).
6.1.4        Operation in the long centrifugation mode:
6.1.4.1  Open the lid and place EVEN number of tubes in rotor sockets opposite one another.
6.1.4.2  Close the lid.
6.1.4.3  Turn the switch into position II to start centrifugation.
6.1.4.4  Turn the switch into position O (OFF) to stop centrifugation.
6.1.5        After the centrifuge machine stops, remove the centrifuge tubes and place in the stand for observations
6.1.6        Disconnect unit from the mains.
6.2  Precautions:
6.2.1        Combi-Spin will not operate at any moment when the lid is opened.
6.2.2        Do not open the lid during work, run-up or shut off of the rotor. The unit stops automatically when the lid is open.
6.2.3        Centrifuge tubes should be places in opposite side in order to balance.
6.2.4        Lid should place over it.
6.2.5        Transfer centrifuge tube without disturbing settled layer.
6.2.6        Always set regulator to position O when you have to stop revolutions. Do not “OFF” it from main switch directly.
6.2.7        If your sample is filled only in one test tube, balance your instrument by placing a centrifuge tube filled with water, in opposite side.
6.2.8        Switch “OFF” and replace the plug from socket, when not in use.
7.0  REVISION LOG:
Revision No.
Effective Date
Reason
00

New SOP


8.0  REFERENCES:
8.1  Not Applicable.
9.0  ANNEXURES:
Annexure 1: Arrangement of centrifuge tube.

Annexure: 1
ARRANGEMENT OF CENTRIFUGE TUBE
Arrangement of centrifuge tube





10.0    ABBREVIATIONS:
Abbreviation
Expanded Form
SOP
Standard operating procedure
&
And
No.
Number  
Ltd.
Limited
Q.C
Quality control
QCEO
Quality control equipment operation
F
Format


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